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Colonisation of the colonic mucus gel layer with butyrogenic and hydrogenotropic bacteria in health and ulcerative colitis

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dc.contributor.author Earley, Helen
dc.contributor.author Lennon, Grainne
dc.contributor.author Coffey, Calvin J.
dc.contributor.author Winter, Desmond C.
dc.contributor.author O'Connell, Ronan
dc.date.accessioned 2021-04-14T08:32:19Z
dc.date.available 2021-04-14T08:32:19Z
dc.date.issued 2021
dc.identifier.uri http://hdl.handle.net/10344/9997
dc.description peer-reviewed en_US
dc.description.abstract Butyrate is the primary energy source for colonocytes and is essential for mucosal integrity and repair. Butyrate deficiency as a result of colonic dysbiosis is a putative factor in ulcerative colitis (UC). Commensal microbes are butyrogenic, while others may inhibit butyrate, through hydrogenotropic activity. The aim of this study was to quantify butyrogenic and hydrogenotropic species and determine their relationship with inflammation within the colonic mucus gel layer (MGL). Mucosal brushings were obtained from 20 healthy controls (HC), 20 patients with active colitis (AC) and 14 with quiescent colitis (QUC). Abundance of each species was determined by RT-PCR. Inflammatory scores were available for each patient. Statistical analyses were performed using Mann–Whitney-U and Kruskall-Wallis tests. Butyrogenic R. hominis was more abundant in health than UC (p< 0.005), prior to normalisation against total bacteria. Hydrogenotropic B. wadsworthia was reduced in AC compared to HC and QUC (p < 0.005). An inverse correlation existed between inflammation and R. hominis (ρ− 0.460, p < 0.005) and B. wadsworthia (ρ− 0.646, p < 0.005). Other hydrogenotropic species did not widely colonise the MGL. These data support a role for butyrogenic bacteria in UC. Butyrate deficiency in UC may be related to reduced microbial production, rather than inhibition by micrrobial by-products. en_US
dc.language.iso eng en_US
dc.publisher Nature en_US
dc.relation.ispartofseries Scientifc Reports;11, 7262
dc.subject butyrogenic en_US
dc.subject hydrogenotropic en_US
dc.title Colonisation of the colonic mucus gel layer with butyrogenic and hydrogenotropic bacteria in health and ulcerative colitis en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1038/s41598-021-86166-6
dc.contributor.sponsor SFI en_US
dc.relation.projectid 09/IN.1/B2606 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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