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In vitro and In vivo effects of palmaria palmata derived peptides on glucose metabolism

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Show simple item record Harnedy-Rothwell, Pádraigín A. McLaughlin, Chris M. Le Gouic, Aurélien V. Mullen, Ciaran Parthsarathy, Vadivel Allsopp, Philip J. McSorley, Emeir M. FitzGerald, Richard J. O’Harte, Finbarr P. M. 2021-04-07T09:20:46Z 2021-04-07T09:20:46Z 2021
dc.description peer-reviewed en_US
dc.description.abstract Three synthetic peptides, ILAP, LLAP and MAGVDHI, derived from a Palmaria palmata protein hydrolysate were assessed for their antidiabetic potential in vitro and in vivo. In addition to inhibiting dipeptidyl peptidase-IV in a cell-based in situ assay all three peptides signifcantly increased the half-life of the incretin hormone glucagon-like peptide-1 (GLP-1). ILAP and LLAP mediated a signifcant increase (p<0.001) in insulin secretion from BRIN-BD11 cells compared to the glucose control, while MAGVDHI had no insulinotropic activity at an eqimolar concentration (10–6 M). A signifcant increase in the concentration of cyclic adenosine monophosphate production in BRIN-BD11 cells mediated by ILAP (p<0.001) and LLAP (p<0.01) compared to the basal control, would indicate that insulin secretion may be mediated by membrane based activation. Furthermore, ILAP and LLAP acted as glucose-dependent insulinotropic polypeptide (GIP) secretagogues, stimulating a signifcant increase (p<0.01) in the concentration of GIP released from enteroendocrine STC-1 cells compared to the glucose control. When tested in vivo in healthy male NIH Swiss mice, ILAP and LLAP, mediated a signifcant increase (p<0.01) in plasma insulin and decrease (p<0.05) in blood glucose, respectively, compared to the control. MAGVDHI mediated a signifcant (p<0.001) sustained reduction in food intake in food deprived trained mice. These results demonstrate that the Palmaria palmata peptides studied herein have prospective antidiabetic activity and have the potential to act as agents that can be used alone or in combination with drugs, to aid in the prevention and management of Type 2 diabetes mellitus. en_US
dc.language.iso eng en_US
dc.publisher Springer en_US
dc.relation.ispartofseries International Journal of Peptide Research and Therapeutics;
dc.subject Bioactive peptides en_US
dc.subject Dipeptidyl peptidase-IV en_US
dc.subject Type 2 diabetes en_US
dc.title In vitro and In vivo effects of palmaria palmata derived peptides on glucose metabolism en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.contributor.sponsor Department of Agriculture, Food and the Marine en_US
dc.contributor.sponsor Northern Ireland Department of Employment and Learning en_US
dc.relation.projectid 13/F/467 en_US
dc.relation.projectid 14/F/873 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US

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