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Sperm selection by rheotaxis improves sperm quality and early embryo development

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Show simple item record Romero-Aguirregomezcorta, Jon Laguna-Barraza, Ricardo Fernández-González, Raúl Štiavnická, Miriama Ward, Fabian Cloherty, Jennifer McAuliffe, Denis Larsen, Peter B Grabrucker, Andreas M. Gutiérrez-Adán, Alfonso Newport, David Fair, Seán 2021-03-26T08:58:42Z 2021-03-26T08:58:42Z 2021
dc.description peer-reviewed en_US
dc.description.abstract The objective of this work was to elucidate whether a sperm selection method that combines rheotaxis and microfluidics can improve the selection of spermatozoa over density gradient and swim-up. For this purpose human sperm selected by rheotaxis were compared against density gradient, swim-up and a control group of non-selected spermatozoa in split frozen-thawed (FT) and fresh (F) semen samples. Sperm quality was assessed in terms of motility, morphology, DNA fragmentation index (DFI), viability, acrosome integrity and membrane fluidity. Using a mouse model, we compared fertilisation and embryo development rates after performing ICSI with spermatozoa, sorted using rheotaxis or swim-up. Selection by rheotaxis yielded a sperm population with reduced DFI than the control (P < 0.05), improved normal morphology (P < 0.001) and higher total motility (TM; P < 0.001) than the other techniques studied in F and FT samples. Swim-up increased TM compared to density gradient and control in FT or F samples (P < 0.001), and yielded lower DFI than the control with F samples (P < 0.05). In FT samples, selection by rheotaxis yielded sperm with higher viability than control, density gradient and swim-up (P < 0.01) while acrosomal integrity and membrane fluidity were maintained. When mouse spermatozoa were selected for ICSI using rheotaxis compared to swim-up, there was an increase in fertilisation (P < 0.01), implantation (P < 0.001) and foetal development rates (P < 0.05). These results suggest that, in the absence of non-destructive DNA testing, the positive rheotaxis can be used to select a population of low DNA fragmentation spermatozoa with high motility, morphology and viability, leading to improved embryo developmental rates. en_US
dc.language.iso eng en_US
dc.publisher Society for Reproduction and Fertility [Associate Organisation] BioScientifica [Commercial Publisher] en_US
dc.relation.ispartofseries Reproduction;161 (3), pp. 343-352
dc.rights This manuscript has been accepted for publication in [insert name of journal], but the version presented here has not yet been copy-edited, formatted or proofed. Consequently, Bioscientifica accepts no responsibility for any errors or omissions it may contain. The final definitive version is available at en_US
dc.subject spermatozoa en_US
dc.subject DNA en_US
dc.title Sperm selection by rheotaxis improves sperm quality and early embryo development en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1530/REP-20-0364
dc.contributor.sponsor EI en_US
dc.relation.projectid CF 2014 4364 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US

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