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A fish-derived protein hydrolysate induces postprandial aminoacidaemia and skeletal muscle anabolism in an in vitro cell model using ex vivo human serum

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dc.contributor.author Lees, Matthew J.
dc.contributor.author Nolan, David
dc.contributor.author Amigo-Benavent, Miryam
dc.contributor.author Raleigh, Conor J
dc.contributor.author Khatib, Neda
dc.contributor.author Harnedy, Pádraigín A.
dc.contributor.author FitzGerald, Richard J.
dc.contributor.author Egan, Brendan
dc.contributor.author Carson, Brian P.
dc.date.accessioned 2021-03-05T09:50:04Z
dc.date.available 2021-03-05T09:50:04Z
dc.date.issued 2021
dc.identifier.uri http://hdl.handle.net/10344/9849
dc.description peer-reviewed en_US
dc.description.abstract Fish-derived proteins, particularly fish protein hydrolysates (FPH), offer potential as high-quality sources of dietary protein, whilst enhancing economic and environmental sustainability. This study investigated the impact of a blue whiting-derived protein hydrolysate (BWPH) on aminoacidaemia in vivo and skeletal muscle anabolism in vitro compared with whey protein isolate (WPI) and an isonitrogenous, non-essential amino acid (NEAA) control (0.33 g·kg−1 body mass−1) in an ex vivo, in vitro experimental design. Blood was obtained from seven healthy older adults (two males, five females; age: 72 ± 5 years, body mass index: 24.9 ± 1.6 kg·m2 ) in three separate trials in a randomised, counterbalanced, double-blind design. C2C12 myotubes were treated with ex vivo human serum-conditioned media (20%) for 4 h. Anabolic signalling (phosphorylation of mTOR, p70S6K, and 4E-BP1) and puromycin incorporation were determined by immunoblotting. Although BWPH and WPI both induced postprandial essential aminoacidaemia in older adults above the NEAA control, peak and area under the curve (AUC) leucine and essential amino acids were more pronounced following WPI ingestion. Insulin was elevated above baseline in WPI and BWPH only, a finding reinforced by higher peak and AUC values compared with NEAA. Muscle protein synthesis, as measured by puromycin incorporation, was greater after incubation with WPI-fed serum compared with fasted serum (P = 0.042), and delta change was greater in WPI (P = 0.028) and BWPH (P = 0.030) compared with NEAA. Myotube hypertrophy was greater in WPI and BWPH compared with NEAA (both P = 0.045), but was similar between bioactive conditions (P = 0.853). Taken together, these preliminary findings demonstrate the anabolic potential of BWPH in vivo and ex vivo, thus providing justification for larger studies in older adults using gold-standard measures of acute and chronic MPS in vivo. en_US
dc.language.iso eng en_US
dc.publisher MDPI en_US
dc.relation.ispartofseries Nutrients;13, 647
dc.subject ageing en_US
dc.subject blue whiting en_US
dc.subject hypertrophy en_US
dc.title A fish-derived protein hydrolysate induces postprandial aminoacidaemia and skeletal muscle anabolism in an in vitro cell model using ex vivo human serum en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.3390/nu13020647
dc.contributor.sponsor Marine Institute Ireland en_US
dc.relation.projectid IND-18-20 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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