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Structural elucidation of Irish ale bioactive polar lipids with antithrombotic properties

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dc.contributor.author Tsoupras, Alexandros
dc.contributor.author Lordan, Ronan
dc.contributor.author O'Keefe, Eoin
dc.contributor.author Shiels, Katie
dc.contributor.author Kumar Saha, Sushanta
dc.contributor.author Zabetakis, Ioannis
dc.date.accessioned 2020-08-04T08:45:49Z
dc.date.available 2020-08-04T08:45:49Z
dc.date.issued 2020
dc.identifier.uri http://hdl.handle.net/10344/9071
dc.description peer-reviewed en_US
dc.description.abstract The structures of bioactive polar lipids (PLs) of Irish ale with potent antithrombotic and cardioprotective properties were elucidated. Ale PL was fractionated by preparative thin layer chromatography (TLC) into subclasses, and their antithrombotic effect was assessed against human platelet aggregation induced by the pro-inflammatory mediator, platelet-activating factor (PAF). The fatty acid content and the overall structures of ale PL were elucidated by liquid chromatography mass spectrometry (LC-MS). Phosphatidylcholines, (PC) and molecules of the sphingomyelin (SM) family exhibited the strongest anti-PAF effects, followed by phosphatidylethanolamines (PE). PC contained higher amounts of omega-3 polyunsaturated fatty acids (n-3 PUFA) and thus the lowest n-6/n-3 ratio. Bioactive diacyl and alkyl-acyl PC and PE molecules bearing n-3 PUFA at their sn-2 position, especially docosahexaenoic acid (DHA) and α-linolenic acid (ALA) but mostly oleic acid (OA), were identified in both PC and PE subclasses. Eicosapentaenoic acid (EPA) was present only in bioactive PC molecules and not in PE, explaining the lower anti-PAF effects of PE. Bioactive sphingolipid and glycolipid molecules with reported anti-inflammatory and anti-tumour properties, such as specific ceramides and glucosylcerebrosides with sphingosine, phytosphingosine and dihydrosphingosine bases but also specific monogalactodiglycerides and SM species bearing ALA at their sn-2 position, were identified in the SM subclass, providing a rational for its strong bioactivities against the PAF pathway. Further studies are required on the health benefits of bioactive PL from beer and brewery by-products. en_US
dc.language.iso eng en_US
dc.publisher MDPI en_US
dc.relation.ispartofseries Biomolecules;10, 1075
dc.subject beer en_US
dc.subject CVD en_US
dc.subject PAF en_US
dc.subject polar lipids en_US
dc.title Structural elucidation of Irish ale bioactive polar lipids with antithrombotic properties en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.3390/biom10071075
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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