University of Limerick Institutional Repository

Frequent traces of EBV infection in Hodgkin and non-Hodgkin lymphomas classified as EBV-negative by routine methods: expanding the landscape of EBV-related lymphoma

DSpace Repository

Show simple item record

dc.contributor.author Mundo, Lucia
dc.contributor.author Del Porro, Leonardo
dc.contributor.author Granai, Massimo
dc.contributor.author Siciliano, Maria Chiara
dc.contributor.author Mancini, Virginia
dc.contributor.author Santi, Raffaella
dc.contributor.author Marcar, Lynnette
dc.contributor.author Vrzalikova, Katerina
dc.contributor.author Vergoni, Federica
dc.contributor.author Di Stefano, Gioia
dc.contributor.author Schiavoni, Gianluca
dc.contributor.author Segreto, Giovanna
dc.contributor.author Onyango, Noel
dc.contributor.author Nyagol, Joshua Akelo
dc.contributor.author Amato, Teresa
dc.contributor.author Bellan, Cristiana
dc.contributor.author Anagnostopoulos, Ioannis
dc.contributor.author Falini, Brunangelo
dc.contributor.author Leoncini, Lorenzo
dc.contributor.author Tiacci, Enrico
dc.contributor.author Lazzi, Stefano
dc.date.accessioned 2020-07-27T11:00:43Z
dc.date.available 2020-07-27T11:00:43Z
dc.date.issued 2020
dc.identifier.uri http://hdl.handle.net/10344/9044
dc.description peer-reviewed en_US
dc.description.abstract The Epstein–Barr virus (EBV) is linked to various B-cell lymphomas, including Burkitt lymphoma (BL), classical Hodgkin lymphoma (cHL) and diffuse large B-cell lymphoma (DLBCL) at frequencies ranging, by routine techniques, from 5 to 10% of cases in DLBCL to >95% in endemic BL. Using higher-sensitivity methods, we recently detected EBV traces in a few EBV-negative BL cases, possibly suggesting a “hit-and-run” mechanism. Here, we used routine and higher-sensitivity methods (qPCR and ddPCR for conserved EBV genomic regions and miRNAs on microdissected tumor cells; EBNA1 mRNA In situ detection by RNAscope) to assess EBV infection in a larger lymphoma cohort [19 BL, 34 DLBCL, 44 cHL, 50 follicular lymphomas (FL), 10 T-lymphoblastic lymphomas (T-LL), 20 hairy cell leukemias (HCL), 10 mantle cell lymphomas (MCL)], as well as in several lymphoma cell lines (9 cHL and 6 BL). qPCR, ddPCR, and RNAscope consistently documented the presence of multiple EBV nucleic acids in rare tumor cells of several cases EBV-negative by conventional methods that all belonged to lymphoma entities clearly related to EBV (BL, 6/9 cases; cHL, 16/32 cases; DLBCL, 11/30 cases), in contrast to fewer cases (3/47 cases) of FL (where the role of EBV is more elusive) and no cases (0/40) of control lymphomas unrelated to EBV (HCL, T-LL, MCL). Similarly, we revealed traces of EBV infection in 4/5 BL and 6/7 HL cell lines otherwise conventionally classified as EBV negative. Interestingly, additional EBV-positive cases (1 DLBCL, 2 cHL) relapsed as EBV-negative by routine methods while showing EBNA1 expression in rare tumor cells by RNAscope. The relapse specimens were clonally identical to their onset biopsies, indicating that the lymphoma clone can largely loose the EBV genome over time but traces of EBV infection are still detectable by high-sensitivity methods. We suggest EBV may contribute to lymphoma pathogenesis more widely than currently acknowledged. en_US
dc.language.iso eng en_US
dc.publisher Springer Nature en_US
dc.relation.ispartofseries Modern Pathology;
dc.relation.uri https://doi.org/10.1038/s41379-020-0575-3
dc.subject EBV infection en_US
dc.title Frequent traces of EBV infection in Hodgkin and non-Hodgkin lymphomas classified as EBV-negative by routine methods: expanding the landscape of EBV-related lymphoma en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1038/s41379-020-0575-3
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search ULIR


Browse

My Account

Statistics