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Human bone marrow stem/stromal cell osteogenesis is regulated via mechanically activated osteocyte-derived extracellular vesicles

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dc.contributor.author Eichholz, Kian F.
dc.contributor.author Woods, Ian
dc.contributor.author Riffault, Mathieu
dc.contributor.author Johnson, Gillian P.
dc.contributor.author Corrigan, Michele
dc.contributor.author Lowry, Michelle C.
dc.contributor.author Shen, Nian
dc.contributor.author Labour, Marie-Noëlle
dc.contributor.author Wynne, Kieran
dc.contributor.author O'Driscoll, Lorraine
dc.contributor.author Hoey, David A.
dc.date.accessioned 2020-07-22T07:34:12Z
dc.date.available 2020-07-22T07:34:12Z
dc.date.issued 2020
dc.identifier.uri http://hdl.handle.net/10344/9024
dc.description peer-reviewed en_US
dc.description.abstract Bone formation or regeneration requires the recruitment, proliferation, and osteogenic differentiation of stem/stromal progenitor cells. A potent stimulus driving this process is mechanical loading. Osteocytes are mechanosensitive cells which play fundamental roles in coordinating loading-induced bone formation via the secretion of paracrine factors. However, the exact mechanisms by which osteocytes relay mechanical signals to these progenitor cells are poorly understood. Therefore, this study aimed to demonstrate the potency of the mechanically stimulated osteocyte secretome in driving human bone marrow stem/stromal cell (hMSC) recruitment and differentiation, and characterize the secretome to identify potential factors regulating stem cell behavior and bone mechanobiology. We demonstrate that osteocytes subjected to fluid shear secrete a distinct collection of factors that significantly enhance hMSC recruitment and osteogenesis and demonstrate the key role of extracellular vesicles (EVs) in driving these effects. This demonstrates the pro-osteogenic potential of osteocyte-derived mechanically activated extracellular vesicles, which have great potential as a cell-free therapy to enhance bone regeneration and repair in diseases such as osteoporosis. en_US
dc.language.iso eng en_US
dc.publisher Wiley en_US
dc.relation GOIPG/2014/493 en_US
dc.relation.ispartofseries Stem Cells Translational Medicine;pp. 1–17
dc.subject bone en_US
dc.subject extracellular vesicle en_US
dc.subject marrow stem cell en_US
dc.subject mechanobiology en_US
dc.title Human bone marrow stem/stromal cell osteogenesis is regulated via mechanically activated osteocyte-derived extracellular vesicles en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1002/sctm.19-0405
dc.contributor.sponsor IRC en_US
dc.contributor.sponsor SFI en_US
dc.contributor.sponsor ERC en_US
dc.relation.projectid IRCLA/2019/49 en_US
dc.relation.projectid 722148 en_US
dc.relation.projectid 825905 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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