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Novel essential amino acid supplements following resistance exercise induce aminoacidemia and enhance anabolic signaling irrespective of age: A proof-of-concept trial

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dc.contributor.author Lees, Matthew J.
dc.contributor.author Wilson, Oliver J.
dc.contributor.author Webb, Erin K.
dc.contributor.author Traylor, Daniel A.
dc.contributor.author Prior, Todd
dc.contributor.author Elia, Antonis
dc.contributor.author Harlow, Paul S.
dc.contributor.author Black, Alistair D.
dc.contributor.author Parker, Paul J.
dc.contributor.author Harris, Nick
dc.contributor.author Cooke, Michael
dc.contributor.author Balchin, Christopher
dc.contributor.author Butterworth, Mathew
dc.contributor.author Phillips, Stuart M.
dc.contributor.author Ispoglo, Theocharis
dc.date.accessioned 2020-07-21T07:58:34Z
dc.date.available 2020-07-21T07:58:34Z
dc.date.issued 2020
dc.identifier.uri http://hdl.handle.net/10344/9016
dc.description peer-reviewed en_US
dc.description.abstract We investigated the e ects of ingesting a leucine-enriched essential amino acid (EAA) gel alone or combined with resistance exercise (RE) versus RE alone (control) on plasma aminoacidemia and intramyocellular anabolic signaling in healthy younger (28 4 years) and older (71 3 years) adults. Blood samples were obtained throughout the three trials, while muscle biopsies were collected in the postabsorptive state and 2 h following RE, following the consumption of two 50 mL EAA gels (40% leucine, 15 g total EAA), and following RE with EAA (combination (COM)). Protein content and the phosphorylation status of key anabolic signaling proteins were determined via immunoblotting. Irrespective of age, during EAA and COM peak leucinemia (younger: 454 32 M and 537 111 M; older: 417 99 M and 553 136 M) occurred ~60–120 min post-ingestion (younger: 66 6 min and 120 60 min; older: 90 13 min and 78 12 min). In the pooled sample, the area under the curve for plasma leucine and the sum of branched-chain amino acids was significantly greater in EAA and COM compared with RE. For intramyocellular signaling, significant main e ects were found for condition (mTOR (Ser2481), rpS6 (Ser235/236)) and age (S6K1 (Thr421/Ser424), 4E-BP1 (Thr37/46)) in age group analyses. The phosphorylation of rpS6 was of similar magnitude (~8-fold) in pooled and age group data 2 h following COM. Our findings suggest that a gel-based, leucine-enriched EAA supplement is associated with aminoacidemia and a muscle anabolic signaling response, thus representing an e ective means of stimulating muscle protein anabolism in younger and older adults following EAA and COM. en_US
dc.language.iso eng en_US
dc.publisher MDPI en_US
dc.relation.ispartofseries Nutrients;12, 2067
dc.subject leucine en_US
dc.subject muscle mass en_US
dc.subject sarcopenia en_US
dc.subject aging en_US
dc.title Novel essential amino acid supplements following resistance exercise induce aminoacidemia and enhance anabolic signaling irrespective of age: A proof-of-concept trial en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.3390/nu12072067
dc.contributor.sponsor American College of Sports Medicine en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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