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Enzymatic modification of porphyra dioica-derived proteins to Improve their antioxidant potential

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dc.contributor.author Pimentel, Filipa B.
dc.contributor.author Cermeño Aínsa, María M.
dc.contributor.author Kleekayai, Thanyaporn
dc.contributor.author Harnedy-Rothwell, Pádraigín A.
dc.contributor.author Fernandes, Eduarda
dc.contributor.author Alves, Rita C.
dc.contributor.author Oliveira, M. Beatriz P.P.
dc.contributor.author FitzGerald, Richard J.
dc.date.accessioned 2020-06-29T10:38:28Z
dc.date.available 2020-06-29T10:38:28Z
dc.date.issued 2020
dc.identifier.uri http://hdl.handle.net/10344/8965
dc.description peer-reviewed en_US
dc.description.abstract Enzymatic hydrolysis has been employed to modify protein functional properties and discover new sources of antioxidants. In this study, the effect of different enzymatic treatments on antioxidant activity of Porphyra dioica (blades and protein isolate (PI)) was investigated. Protein nitrogen content of P. dioica blades and PI were 23 and 50% (dry weight), respectively. Blades and PI were hydrolyzed with Prolyve®and Prolyve®plus Flavourzyme®. Peptide profiles and molecular mass distribution of the hydrolysates were investigated. The hydrolysis promoted generation of peptides and low molecular mass components <1 kDa. Antioxidant activity was assessed using ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH ) scavenging, 2,20-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS +) inhibition, and reactive oxygen species scavenging ability, i.e., oxygen radical absorbance capacity (ORAC) and hypochlorous acid (HOCl) scavenging assays. In general, enzymatic hydrolysis of P. dioica blades and PI enhanced the in vitro antioxidant activity. Direct hydrolysis of blades improved ORAC values up to 5-fold (from 610 to 3054 mol Trolox eq./g freeze dried sample (FDS). The simultaneous release of phenolic compounds suggested a potential synergistic activity (ORAC and ABTS + assays). Such hydrolysates may be of value as functional food ingredients. en_US
dc.language.iso eng en_US
dc.publisher MDPI en_US
dc.relation UIDB/50006/2020 en_US
dc.relation.ispartofseries Molecules;25, 2838
dc.subject Porphyra dioica en_US
dc.subject antioxidant activity en_US
dc.subject enzyme-assisted hydrolysis en_US
dc.subject seaweed en_US
dc.title Enzymatic modification of porphyra dioica-derived proteins to Improve their antioxidant potential en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.3390/molecules25122838
dc.contributor.sponsor Marine Institute en_US
dc.relation.projectid MI:PBA/MB/16/01 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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