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Complete aggregation pathway of amyloid β (1-40) and (1-42) resolved on an atomically clean interface

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dc.contributor.author Nirmalraj, Peter N.
dc.contributor.author List, Jonathan
dc.contributor.author Battacharya, Shayon
dc.contributor.author Howe, Geoffrey
dc.contributor.author Xu, Liang
dc.contributor.author Thompson, Damien
dc.contributor.author Mayer, Michael
dc.date.accessioned 2020-04-24T10:07:02Z
dc.date.available 2020-04-24T10:07:02Z
dc.date.issued 2020
dc.identifier.uri http://hdl.handle.net.proxy.lib.ul.ie/10344/8758
dc.description peer-reviewed en_US
dc.description.abstract To visualize amyloid β (Aβ) aggregates requires an uncontaminated and artifact-free interface. This paper demonstrates the interface between graphene and pure water (verified to be atomically clean using tunneling microscopy) as an ideal platform for resolving size, shape, and morphology (measured by atomic force microscopy) of Aβ-40 and Aβ-42 peptide assemblies from 0.5 to 150 hours at a 5-hour time interval with single-particle resolution. After confirming faster aggregation of Aβ-42 in comparison to Aβ-40, a stable set of oligomers with a diameter distribution of ~7 to 9 nm was prevalently observed uniquely for Aβ-42 even after fibril appearance. The interaction energies between a distinct class of amyloid aggregates (dodecamers) and graphene was then quantified using molecular dynamics simulations. Last, differences in Aβ-40 and Aβ-42 networks were resolved, wherein only Aβ-42 fibrils were aligned through lateral interactions over micrometer-scale lengths, a property that could be exploited in the design of biofunctional materials. en_US
dc.language.iso eng en_US
dc.publisher American Association for the Advancement of Science en_US
dc.relation 200021-169304 en_US
dc.relation.ispartofseries Sciences Advances;6,(15) eaaz6014
dc.subject amyloid en_US
dc.subject artifact-free en_US
dc.subject biofunctiona en_US
dc.title Complete aggregation pathway of amyloid β (1-40) and (1-42) resolved on an atomically clean interface en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1126/sciadv.aaz6014
dc.contributor.sponsor Merkle Foundation en_US
dc.contributor.sponsor SFI en_US
dc.relation.projectid 15/CDA/3491 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2952456


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