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Specific ion effects on the enzymatic activity of alcohol dehydrogenase from Saccharomyces cerevisiae

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dc.contributor.author Carucci, Cristina
dc.contributor.author Raccis, Raccis
dc.contributor.author Salis, Andrea
dc.contributor.author Magner, Edmond
dc.date.accessioned 2020-04-22T10:04:07Z
dc.date.issued 2020
dc.identifier.issn 1463-9084
dc.identifier.uri http://hdl.handle.net/10344/8753
dc.description peer-reviewed en_US
dc.description.abstract The enzymatic activity of alcohol dehydrogenase (ADH) in the presence of a range of electrolytes is investigated. In the presence of 150 and 200 mM cations a substantial increase in activity following the series GnCl no salt > NaClO4 > NaSCN with a peak in activity increase of 75% in the presence of NaBr. The values of the Michaelis-Menten constant (Km) did not show any significant ion specific effect, while the maximum rate (Vmax) of ethanol oxidation to acetaldehyde was strongly ion specific. The changes in specific activity and Vmax in the presence of anions likely arises from ion specific interactions with charged residues in the active site of ADH. The data indicate that the enzymatic activity of alcohol dehydrogenase can be modulated by the nature of electrolytes at physiological concentration. en_US
dc.language.iso eng en_US
dc.publisher Royal Society of Chemistry en_US
dc.relation 13RC2073 en_US
dc.relation.ispartofseries Physical Chemistry Chemical Physics;22 (12), pp 6749-6754
dc.rights Royal Society of Chemistry © 2020. Personal use of this material is permitted. Permission from Royal Society of Chemistry must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works en_US
dc.subject alcohol dehydrogenase (ADH) en_US
dc.title Specific ion effects on the enzymatic activity of alcohol dehydrogenase from Saccharomyces cerevisiae en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.date.updated 2020-04-22T09:54:53Z
dc.description.version ACCEPTED
dc.identifier.doi 10.1039/c9cp06800g
dc.contributor.sponsor SFI en_US
dc.relation.projectid 13/RC/2073 en_US
dc.relation.projectid FIR 2019 en_US
dc.relation.projectid FFABR 2017 en_US
dc.date.embargoEndDate 2021-03-09
dc.embargo.terms 2021-03-09 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2949864
dc.internal.copyrightchecked Yes
dc.identifier.journaltitle Physical Chemistry Chemical Physics
dc.description.status peer-reviewed


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