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Zymobacter palmae pyruvate decarboxylase is Less effective than that of zymomonas mobilis for ethanol production in metabolically engineered synechocystis sp. PCC6803

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dc.contributor.author Quinn, Lorraine
dc.contributor.author Armshaw, Patricia
dc.contributor.author Soulimane, Tewfik
dc.contributor.author Sheehan, Con
dc.contributor.author Ryan, Michael P.
dc.contributor.author Pembroke, Tony J.
dc.date.accessioned 2019-12-05T10:02:59Z
dc.date.available 2019-12-05T10:02:59Z
dc.date.issued 2019
dc.identifier.uri http://hdl.handle.net/10344/8297
dc.description peer-reviewed en_US
dc.description.abstract produce bioethanol from model cyanobacteria such as Synechocystis, a two gene cassette consisting of genes encoding pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) are required to transform pyruvate first to acetaldehyde and then to ethanol. However the partition of pyruvate to ethanol comes at a cost, a reduction in biomass and pyruvate availability for other metabolic processes. Hence strategies to divert flux to ethanol as a biofuel in Synechocystis are of interest. PDC from Zymobacter palmae (ZpPDC) has been reported to have a lower Km then the Zymomonas mobilis PDC (ZmPDC), which has traditionally been used in metabolic engineering constructs. The Zppdc gene was combined with the native slr1192 alcohol dehydrogenase gene (adhA) in an attempt to increase ethanol production in the photoautotrophic cyanobacterium Synechocystis sp. PCC 6803 over constructs created with the traditional Zmpdc. Native (Zppdc) and codon optimized (ZpOpdc) versions of the ZpPDC were cloned into a construct where pdc expression was controlled via the psbA2 light inducible promoter from Synechocystis sp. PCC 6803. These constructs were transformed into wildtype Synechocystis sp. PCC 6803 for expression and ethanol production. Ethanol levels were then compared with identical constructs containing the Zmpdc. While strains with the Zppdc (UL071) and ZpOpdc (UL072) constructs did produce ethanol, levels were lower compared to a control strain (UL070) expressing the pdc from Zymomonas mobilis. All constructs demonstrated lower biomass productivity illustrating that the flux from pyruvate to ethanol has a major e ect on biomass and ultimately overall biofuel productivity. Thus the utilization of a PDC with a lower Km from Zymobacter palmae unusually did not result in enhanced ethanol production in Synechocystis sp. PCC 6803. en_US
dc.language.iso eng en_US
dc.publisher MDPI en_US
dc.relation FP7 DEMA en_US
dc.relation.ispartofseries Microorganisms;7, 494
dc.subject alcohol dehydrogenase en_US
dc.subject biofuels en_US
dc.subject cyanobacteria en_US
dc.subject ethanol en_US
dc.subject renewable energy en_US
dc.title Zymobacter palmae pyruvate decarboxylase is Less effective than that of zymomonas mobilis for ethanol production in metabolically engineered synechocystis sp. PCC6803 en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.3390/microorganisms7110494
dc.contributor.sponsor ERC en_US
dc.relation.projectid 309086 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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