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Progesterone induces the release of bull spermatozoa from oviductal epithelial cells

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Show simple item record Romero-Aguirregomezcorta, Jon Cronin S. Donnellan, E.M. Fair, Seán 2019-11-07T10:11:59Z 2019-11-07T10:11:59Z 2019
dc.identifier.citation Romero-Aguirregomezcorta J.;Cronin S.;Donnellan E.;Fair S. (2019) 'Progesterone induces the release of bull spermatozoa from oviductal epithelial cells'. Reproduction Fertility And Development, . en_US
dc.identifier.issn 1031-3613
dc.description peer-reviewed en_US
dc.description.abstract The mechanism that causes the detachment of spermatozoa from the oviductal reservoir around the time of ovulation remains to be elucidated. Because the cumulus cells of the bovine oocyte are known to secrete progesterone (P4), and P4 has been shown to act upon cation channels of spermatozoa (CatSper) in human spermatozoa, it was hypothesised that P4 could induce hyperactivation due to an influx of extracellular calcium, and this would facilitate detachment of spermatozoa from oviductal epithelial cells. Therefore, this study aimed to investigate the role and mechanism of action of P4 in the release of spermatozoa from bovine oviduct epithelial cells (BOEC). Initial dose–response assessments on sperm hyperactivation determined the optimum concentration of P4 (10 nM), mibefradil (a non-specific Ca2+ channel antagonist; 5 µM), NNC 55-0396 dihydrochloride (NNC; a CatSper antagonist; 2 µM), mifepristone (a classical and membrane P4 receptor antagonist; 400 nM) and AG205 (a membrane P4 receptor antagonist; 10 μM). BOEC explants were incubated with frozen–thawed bovine spermatozoa for 30 min, following which loosely bound spermatozoa were removed. Two experiments were completed. In Experiment 1, BOECs were treated for 30 min with either no treatment, P4, NNC, mibefradil, P4 + mibefradil, P4 + NNC, P4 + mibefradil + NNC or P4 + EGTA. In Experiment 2, BOECs were treated for 30 min with either no treatment, P4, mifepristone, AG205, mifepristone + AG205, P4 + mifepristone, P4 + AG205 or P4 + mifepristone + AG205. The number of spermatozoa remaining bound per millimetre squared of BOEC explant was determined. Progesterone stimulated the release of bound spermatozoa from BOEC explants, whereas NNC, mibefradil and EGTA inhibited this release. The release of spermatozoa by P4 was inhibited in the presence of both mifepristone and AG205, whereas the combination of both had the greatest inhibitory action on P4 release of spermatozoa. These findings suggest the presence of a P4 membrane receptor on bovine spermatozoa and that P4-induced release of spermatozoa from BOECs is likely mediated by extracellular Ca2+. en_US
dc.language.iso eng en_US
dc.publisher CSIRO Publishing en_US
dc.relation 16IA4474 en_US
dc.relation.ispartofseries Reproduction, Fertility and Development;31 (9), pp. 1463-1472
dc.subject membrane progesterone receptor en_US
dc.subject oviduct en_US
dc.subject progesterone receptor membrane component 1 (PGRMC1) en_US
dc.subject unbinding en_US
dc.title Progesterone induces the release of bull spermatozoa from oviductal epithelial cells en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US 2019-11-07T10:01:26Z
dc.description.version ACCEPTED
dc.identifier.doi 10.1071/RD18316
dc.contributor.sponsor SFI en_US
dc.relation.projectid 16/IA/4474 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2904708
dc.internal.copyrightchecked Yes
dc.identifier.journaltitle Reproduction Fertility And Development
dc.description.status peer-reviewed

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