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Investigating the thermostability of succinate: quinone oxidoreductase enzymes by direct electrochemistry at SWNTs-modified electrodes and FTIR spectroscopy

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dc.contributor.author Melin, Frederic
dc.contributor.author Noor, Mohamed R.
dc.contributor.author Pardieu, Elodie
dc.contributor.author Boulmedais, Fouzia
dc.contributor.author Banhart, Florian
dc.contributor.author Cecchini, Gary
dc.contributor.author Soulimane, Tewfik
dc.contributor.author Hellwig, Petra
dc.date.accessioned 2019-10-31T12:30:11Z
dc.date.available 2019-10-31T12:30:11Z
dc.date.issued 2014
dc.identifier.uri http://hdl.handle.net/10344/8195
dc.description peer-reviewed en_US
dc.description.abstract Succinate Quinone reductases (SQRs) are the enzymes which couple the oxidation of succinate and the reduction of quinones in the respiratory chain of prokaryotes and eukaryotes. We compare herein the temperature-dependent activity and structural stability of two SQRs, the first one from the mesophilic bacterium E. coli and the second one from the thermophilic bacterium T. thermophilus by a combined electrochemical and infrared spectroscopy approach. Direct electron transfer was achieved with the full membrane protein complexes at SWNTs-modified electrodes. The possible structural factors which contribute to the temperature-dependent activity of the enzymes and to the thermostability of the T. thermophiles SQR in particular, are discussed. en_US
dc.language.iso eng en_US
dc.publisher Wiley and Sons Ltd en_US
dc.relation.ispartofseries Chemphyschem;15 (16),pp. 3572-3579
dc.relation.uri https://doi.org/10.1002/cphc.201402354
dc.rights This is the author's version of the following article: Investigating the thermostability of succinate: quinone oxidoreductase enzymes by direct electrochemistry at SWNTs-modified electrodes and FTIR spectroscopy, published in Chemphyschem, 2014, 15 (16), pp. 3572-3579The definitive version is available at https://doi.org/10.1002/cphc.201402354. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. http://olabout.wiley.com/WileyCDA/Section/id-828039.html#terms en_US
dc.subject membrane proteins en_US
dc.subject succinate en_US
dc.subject quinone en_US
dc.subject oxidoreductase en_US
dc.subject carbon nontubes en_US
dc.subject protein film voltammetry en_US
dc.subject IR spectroscopy en_US
dc.subject thermostability en_US
dc.title Investigating the thermostability of succinate: quinone oxidoreductase enzymes by direct electrochemistry at SWNTs-modified electrodes and FTIR spectroscopy en_US
dc.type info:eu-repo/semantics/article
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1002/cphc.201402354
dc.contributor.sponsor International Center for Frontier Research in Chemistry in Strasbourg (RTRA) en_US
dc.contributor.sponsor Institut Universitaire de France (IUF) en_US
dc.contributor.sponsor IRC en_US
dc.relation.projectid BX001077 en_US
dc.relation.projectid GM61606 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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