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Electrochemical detection of Pseudomonas aeruginosa quorum sensing molecules at a liquid | liquid interface

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dc.contributor.author Burgoyne, Edward D.
dc.contributor.author Stockmann, Talia Jane
dc.contributor.author Molina-Osorio, Andrés
dc.contributor.author Shanahan, Rachel
dc.contributor.author McGlacken, Gerard
dc.contributor.author Scanlon, Micheál D.
dc.date.accessioned 2019-10-10T10:55:53Z
dc.date.issued 2019
dc.identifier.uri http://hdl.handle.net/10344/8129
dc.description peer-reviewed en_US
dc.description The full text of this article will not be available in ULIR until the embargo expires on the 12/09/2020
dc.description.abstract Opportunistic pathogenic bacteria, such as Pseudomonas aeruginosa, pose a serious risk to patients suffering from a compromised immune system and those patients with cystic fibrosis. Confirming their presence often requires culturing the bacteria which can take days. Herein is proposed a rapid electrochemical detection method based on P. aeruginosa generated small molecules employed for chemical communication – referred to as quorum sensing (QS) molecules – within the biofilm formed by the bacteria; specifically, 4‐hydroxy‐2‐heptylquinoline (HHQ) and 2‐heptyl‐3,4‐dihydroxyquinoline (PQS, Pseudomonas quinolone signal). This method does not depend on the redox activity of the QS molecules. Instead, as a proof-of-concept, electrochemical monitoring was achieved through aqueous alkali metal ion and proton interfacial complexation with organic solubilized HHQ and PQS at an interface between two immiscible electrolytic solutions (ITIES), specifically, between water and 1,2-dichloroethane. The proton:HHQ and proton:PQS binding stoichiometry’s were discovered to be 1:3 and 1:2, respectively, which is likely due to the relatively high concentrations of QS molecules employed. Owing to the biphasic nature of the methodology, experimental complications due to the poor solubility of the hydrophobic QS molecules in aqueous media were avoided en_US
dc.language.iso eng en_US
dc.publisher American Chemical Society en_US
dc.relation 716792 en_US
dc.relation.ispartofseries Journal of Physical Chemistry C;123 (40), pp. 24643-24650
dc.relation.uri http://dx.doi.org/10.1021/acs.jpcc.9b08350
dc.rights © 2019 ACS This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Physical Chemistry C, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.jpcc.9b08350 en_US
dc.subject pathogenic bacteria en_US
dc.subject Pseudomonas aeruginosa en_US
dc.title Electrochemical detection of Pseudomonas aeruginosa quorum sensing molecules at a liquid | liquid interface en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1021/acs.jpcc.9b08350
dc.contributor.sponsor ERC en_US
dc.contributor.sponsor SFI en_US
dc.contributor.sponsor IRC en_US
dc.contributor.sponsor NSERC en_US
dc.relation.projectid 716792 en_US
dc.relation.projectid 13/SIRG/2137 en_US
dc.relation.projectid GOIPG/2016/1217 en_US
dc.relation.projectid 12/IP/1315 en_US
dc.relation.projectid 12/TIDA/B2405 en_US
dc.relation.projectid 12/RC/2275 en_US
dc.relation.projectid #2019-06074 en_US
dc.date.embargoEndDate 2020-09-12
dc.embargo.terms 2020-09-12 en_US
dc.rights.accessrights info:eu-repo/semantics/embargoedAccess en_US
dc.internal.rssid 2930615


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