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Differential stimulation of post-exercise myofibrillar protein synthesis in humans following isonitrogenous, isocaloric pre-exercise feeding

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dc.contributor.author Davies, Robert W.
dc.contributor.author Bass, Joseph J.
dc.contributor.author Carson, Brian P.
dc.contributor.author Norton, Catherine
dc.contributor.author Kozior, Marta
dc.contributor.author Amigo-Benavent, Miryam
dc.contributor.author Wilkinson, Daniel J.
dc.contributor.author Brook, Matthew S.
dc.contributor.author Atherton, Philip J.
dc.contributor.author Smith, Kenneth
dc.contributor.author Jakeman, Philip M.
dc.date.accessioned 2019-08-22T10:58:26Z
dc.date.available 2019-08-22T10:58:26Z
dc.date.issued 2019
dc.identifier.uri http://hdl.handle.net/10344/8000
dc.description peer-reviewed en_US
dc.description.abstract .The aim of this study was to test the effects of two disparate isonitrogenous, isocaloric pre-exercise feeds on deuterium-oxide (D2O) derived measures of myofibrillar protein synthesis (myoPS) in humans. Methods: In a double-blind parallel group design, 22 resistance-trained men aged 18 to 35 years ingested a meal (6 kcal·kg−1 , 0.8 g·kg−1 carbohydrate, 0.2 g·kg−1 fat) with 0.33 g·kg−1 nonessential amino acids blend (NEAA) or whey protein (WHEY), prior to resistance exercise (70% 1RM back-squats, 10 reps per set to failure, 25% duty cycle). Biopsies of M. vastus lateralis were obtained pre-ingestion (PRE) and +3 h post-exercise (POST). The myofibrillar fractional synthetic rate (myoFSR) was calculated via deuterium labelling of myofibrillar-bound alanine, measured by gas chromatography–pyrolysis–isotope ratio mass spectrometry (GC-Pyr-IRMS). Data are a mean percentage change (95% CI). Results: There was no discernable change in myoFSR following NEAA (10(−5, 25) %, p = 0.235), whereas an increase in myoFSR was observed after WHEY (28 (13, 43) %, p = 0.003). Conclusions: Measured by a D2O tracer technique, a disparate myoPS response was observed between NEAA and WHEY. Pre-exercise ingestion of whey protein increased post-exercise myoPS, whereas a NEAA blend did not, supporting the use of NEAA as a viable isonitrogenous negative control. en_US
dc.language.iso eng en_US
dc.publisher MDPI en_US
dc.relation.ispartofseries Nutrients;11, 1657
dc.subject amino acids en_US
dc.subject deuterium oxide en_US
dc.subject protein synthesis en_US
dc.subject humans en_US
dc.subject whey proteins en_US
dc.title Differential stimulation of post-exercise myofibrillar protein synthesis in humans following isonitrogenous, isocaloric pre-exercise feeding en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.3390/nu11071657
dc.contributor.sponsor EI en_US
dc.relation.projectid TC2013-0001 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2933825


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