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Extraction and purification of curcuminoids from crude curcumin by a combination of crystallization and chromatography

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dc.contributor.author Heffernan, Claire
dc.contributor.author Ukrainczyk, Marko
dc.contributor.author Gamidi, Rama Krishna
dc.contributor.author Hodnett, Benjamin K.
dc.contributor.author Rasmuson, Åke C.
dc.date.accessioned 2019-05-24T08:38:37Z
dc.date.available 2019-05-24T08:38:37Z
dc.date.issued 2017
dc.identifier.uri http://hdl.handle.net/10344/7856
dc.description peer-reviewed en_US
dc.description.abstract In this work a method is developed for the extraction and purification of the three curcuminoids; Curcumin (CUR), Demethoxycurcumin (DMC) and Bisdemethoxycurcumin (BDMC) from commercially available crude curcumin. In a previous publication the extraction of pure curcumin by repeated crystallization has been described. The focus of this paper is on the following chromatographic treatment of the mother liquor from the crystallization to obtain pure DMC and BDMC, and to increase the yield of pure CUR. In the chromatographic process a mixture of chloroform and methanol is used as the mobile phase and silica gel is used as the stationary phase. Each fraction isolated in the chromatographic process was characterised by High Performance Liquid Chromatography (HPLC) and Mass Spectrometry (LC-MS) techniques, and the pure CUR, DMC and BDMC solid phases were fully characterized by powder Xray diffraction (PXRD), differential scanning calorimetry (DSC) and thermal gravimetric analysis (TGA). Stability studies were performed on the purified curcuminoids where the degradation products were observed and analysed by HPLC/LC-MS. Overall, the combined purification method recovered from the crude: 88.5%, 79.7% and 68.8% of CUR, DMC and BDMC respectively, in highly pure form CUR (100%), DMC (98.6%) and BDMC (98.3%). en_US
dc.language.iso eng en_US
dc.publisher American Chemical Society en_US
dc.relation 12RC2275 en_US
dc.relation.ispartofseries Organic Process Research and Development;21 (6), pp. 821-826
dc.relation.uri https://doi.org/10.1021/acs.oprd.6b00347
dc.rights © Year ACS This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal Title, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.oprd.6b00347 en_US
dc.subject purification en_US
dc.subject curcuminoids en_US
dc.subject gram scale en_US
dc.subject cooling Crystallization en_US
dc.subject column chromatography en_US
dc.subject HPLC analysis en_US
dc.title Extraction and purification of curcuminoids from crude curcumin by a combination of crystallization and chromatography en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1021/acs.oprd.6b00347
dc.contributor.sponsor SFI en_US
dc.relation.projectid 12/rc/2275 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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