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Immunomodulatory activity of 5 kDa permeate fractions of casein hydrolysates generated using a range of enzymes in Jurkat T cells and RAW264.7 macrophages

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dc.contributor.author O'Sullivan, Siobhan M.
dc.contributor.author O'Callaghan, Yvonne C.
dc.contributor.author O'Keeffe, Martina B.
dc.contributor.author Fitzgerald, Richard J.
dc.contributor.author O'Brien, Nora M.
dc.date.accessioned 2019-03-08T11:55:33Z
dc.date.issued 2019
dc.identifier.uri http://hdl.handle.net/10344/7670
dc.description peer-reviewed en_US
dc.description.abstract The in vitro bioactivity of 5 kDa ultrafiltration permeate fractions of casein hydrolysates produced using different enzymes were compared. Reverse phase ultra-performance liquid chromatography and gel permeation chromatography showed that the permeates had different physicochemical properties (molecular mass and degree of hydrolysis). The Flavourzyme® permeate had the highest activity in the 2,2’-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay. Cellular antioxidant and immunomodulatory assays showed that none of the permeates exhibited in vitro antioxidant activity, while all permeates significantly (P < 0.05) decreased interleukin-6 (IL-6) production in ConA-stimulated Jurkat T cells at 0.50% (w/v) and LPS-stimulated RAW264.7 cells at 0.05 and 0.50% (w/v). Three permeates, obtained using Flavourzyme®, Flavorpro Whey and trypsin, also significantly (P < 0.05) decreased IL-1β production at 0.05% (w/v) in RAW264.7 cells. Western blot analysis showed that all permeates significantly decreased the expression of the NF-κB subunit, p65, in RAW264.7 cells indicating that anti-inflammatory activity may be associated with this pathway. en_US
dc.language.iso eng en_US
dc.publisher Elseviier en_US
dc.relation.ispartofseries Interntational Dairy Journal;91, pp.9-17
dc.relation.uri https://doi.org/10.1016/j.idairyj.2018.12.005
dc.rights This is the author’s version of a work that was accepted for publication in International Dairy Journal. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in International Dairy Journal, 91, pp. 9-17, https://doi.org/10.1016/j.idairyj.2018.12.005 en_US
dc.subject cardiovascular disease (CVD) en_US
dc.subject liquid chromatography en_US
dc.title Immunomodulatory activity of 5 kDa permeate fractions of casein hydrolysates generated using a range of enzymes in Jurkat T cells and RAW264.7 macrophages en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1016/j.idairyj.2018.12.005
dc.contributor.sponsor Irish Department of Agriculture, Food and the Marine en_US
dc.relation.projectid 11F063 en_US
dc.date.embargoEndDate 2019-12-31
dc.embargo.terms 2019-12-31 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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