University of Limerick Institutional Repository

Purification and characterization of native human elongation factor 2

DSpace Repository

Show simple item record

dc.contributor.author Flygaard, Rasmus Kock
dc.contributor.author Malacrida, Beatrice
dc.contributor.author Kiely, Patrick A.
dc.contributor.author Jenner, Lasse Bohl
dc.date.accessioned 2019-02-22T14:27:27Z
dc.date.issued 2019
dc.identifier.uri http://hdl.handle.net/10344/7616
dc.description peer-reviewed en_US
dc.description.abstract Human elongation factor 2 is the translocase that is responsible for the movement of tRNA from the A- to P- and P- to E-site on the ribosome during the elongation phase of translation. Being a vital factor of protein biosynthesis, its function is highly controlled and regulated. It has been implicated in numerous diseases and pathologies, and as such it is important to have a source for isolated pure and active protein for biomedical and biochemical studies. Here we report development of a purification protocol for native human elongation factor 2 from HEK-293S cells. The resulting protein is active, pure, has an intact diphtamide and is obtainable in yields suitable for functional and structural studies. en_US
dc.language.iso eng en_US
dc.publisher Elsevier en_US
dc.relation.ispartofseries Protein Expression and Purification;158, pp. 15-19
dc.relation.uri https://doi.org/10.1016/j.pep.2019.02.005
dc.rights This is the author’s version of a work that was accepted for publication in Protein Expression and Purification . Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Protein Expression and Purification, 2019, 158, pp. 159-19, https://doi.org/10.1016/j.pep.2019.02.005 en_US
dc.subject human eEF2 en_US
dc.subject translation en_US
dc.title Purification and characterization of native human elongation factor 2 en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1016/j.pep.2019.02.005
dc.date.embargoEndDate 2020-02-08
dc.embargo.terms 2020-02-08 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search ULIR


Browse

My Account

Statistics