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Molecular characterisation of equine herpesvirus 1 isolates from cases of abortion, respiratory and neurological disease in Ireland between 1990 and 2017

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dc.contributor.author Garvey, Marie
dc.contributor.author Lyons, Rachel
dc.contributor.author Hector, Ralph D.
dc.contributor.author Walsh, Cathal Dominic
dc.contributor.author Arkins, Sean
dc.contributor.author Cullinane, Ann
dc.date.accessioned 2019-01-24T12:17:58Z
dc.date.available 2019-01-24T12:17:58Z
dc.date.issued 2019
dc.identifier.uri http://hdl.handle.net/10344/7507
dc.description peer-reviewed en_US
dc.description.abstract Multiple locus typing based on sequencing heterologous regions in 26 open reading frames (ORFs) of equine herpesvirus 1 (EHV-1) strains Ab4 and V592 was used to characterise 272 EHV-1 isolates from 238 outbreaks of abortion, respiratory or neurological disease over a 28-year period. The analysis grouped the 272 viruses into at least 10 of the 13 unique long region (UL) clades previously recognised. Viruses from the same outbreak had identical multi-locus profiles. Sequencing of the ORF68 region of EHV-1 isolates from 222 outbreaks established a divergence into seven groups and network analysis demonstrated that Irish genotypes were not geographically restricted but clustered with viruses from all over the world. Multi-locus analysis proved a more comprehensive method of strain typing than ORF68 sequencing. It was demonstrated that when interpreted in combination with epidemiological data, this type of analysis has a potential role in tracking virus between premises and therefore in the implementation of targeted control measures. Viruses from 31 of 238 outbreaks analysed had the proposed ORF30 G2254/D752 neuropathogenic marker. There was a statistically significant association between viruses of the G2254/D752 genotype and both neurological disease and hypervirulence as defined by outbreaks involving multiple abortion or neurological cases. The association of neurological disease in those with the G2254/D752 genotype was estimated as 27 times greater than in those with the A2254/N752 genotype. en_US
dc.language.iso eng en_US
dc.publisher MDPI en_US
dc.relation EBPPG/2014/62 en_US
dc.relation.ispartofseries Pathogens;8, 7
dc.relation.uri http://dx.doi.org/10.3390/pathogens8010007
dc.subject EHV-1 en_US
dc.subject multi-locus typing en_US
dc.subject ORF68 en_US
dc.subject neuropathogenic marker en_US
dc.title Molecular characterisation of equine herpesvirus 1 isolates from cases of abortion, respiratory and neurological disease in Ireland between 1990 and 2017 en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.3390/pathogens8010007
dc.contributor.sponsor IRC en_US
dc.contributor.sponsor Department of Agriculture, Food and the Marine, Ireland. en_US
dc.relation.projectid EBPPG/2014/62 to MG en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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