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Optimizing storage temperature of liquid bovine semen diluted in INRA96

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dc.contributor.author Murphy, Edel M.
dc.contributor.author O'Meara, Ciara M.
dc.contributor.author Eivers, Bernard
dc.contributor.author Lonergan, Patrick
dc.contributor.author Fair, Seán
dc.date.accessioned 2019-01-21T12:40:32Z
dc.date.issued 2018
dc.identifier.uri http://hdl.handle.net/10344/7503
dc.description peer-reviewed en_US
dc.description.abstract Temperature regulation of liquid bovine semen can be difficult in field situations. Two experiments were carried out to assess the effect of storage temperature on in vitro sperm characteristics and 60-d nonreturn rate (NRR) following artificial insemination (AI) of liquid bovine semen. In experiment 1, the effect of storage of liquid bovine semen in INRA96 diluent (IMV Technologies, L'Aigle, France) at 1 of 5 storage temperatures (5, 15, or 28°C, and fluctuating between 5 and 15°C or 5 and 28°C) on total and progressive motility and kinematic parameters was assessed objectively via computer-assisted sperm analyzer on d 0, 1, 2, 3, and 4 after collection. Fluctuating temperatures were designed to mimic day- to nighttime variation. In experiment 2, we assessed the field fertility of liquid semen stored at a constant 5 or 15°C or in an unregulated manner and compared with that of frozen-thawed semen (total of n = 106,738 inseminations). In experiment 1, we detected a linear decrease in motility with increased duration of storage. Semen stored at a constant 15°C or fluctuating between 5 and 15°C had greater total motility than semen held at 5 or 28°C or fluctuating between 5 and 28°C; however, semen stored at 15°C and fluctuating between 5 and 15°C did not differ from each other. Semen held at a constant 5 or 15°C or fluctuating between 5 and 15°C, although not differing from each other, had higher progressive motility scores than that held at 28°C or fluctuating between 5 and 28°C. Semen stored at a constant 28°C exhibited poor motility and velocity values but had high progressive motion values compared with that all other storage temperatures; however, the other storage temperatures did not differ from each other in relation to motility kinematics. In experiment 2, semen stored at a constant 5°C resulted in a lower 60-d NRR (62.5%) than storage at constant 15°C or unregulated temperature or frozen-thawed semen (73.6, 74.6, and 74.4%, respectively. In conclusion, sperm stored in IRNA96 are quite tolerant in terms of storage temperature, retaining acceptable motility between 5 and 15°C. Storing semen at a constant 15°C resulted in greater in vitro sperm motility and higher NRR rates than storage at 5°C and did not differ in NRR from frozen-thawed semen or semen stored at an unregulated temperature; however, lower storage temperatures were shown to be more detrimental to sperm in vivo than unregulated storage conditions. en_US
dc.language.iso eng en_US
dc.publisher Elsevier en_US
dc.relation.ispartofseries Journal of Dairy Science;101 (6), pp. 5549-5558
dc.relation.uri https://doi.org/10.3168/jds.2017-14205
dc.rights This is the author’s version of a work that was accepted for publication in Journal of Dairy Science. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Dairy Science, 2018, 101 (6), pp, 5549-5558, https://doi.org/10.3168/jds.2017-14205 en_US
dc.subject sperm en_US
dc.subject storage temperature en_US
dc.subject liquid semen en_US
dc.subject bovine en_US
dc.subject artificial insemination en_US
dc.subject non-return rate en_US
dc.title Optimizing storage temperature of liquid bovine semen diluted in INRA96 en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.3168/jds.2017-14205
dc.contributor.sponsor IRC en_US
dc.contributor.sponsor Department of Agriculture, Food and the Marine en_US
dc.contributor.sponsor Teagasc en_US
dc.relation.projectid EBPPG/2014/60 en_US
dc.date.embargoEndDate 2019-03-15
dc.embargo.terms 2019-03-15 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2868354


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