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Effect of increasing equilibration time of diluted bull semen up to 72 h prior to freezing on sperm quality parameters and calving rate following artificial insemination

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dc.contributor.author Murphy, Edel M.
dc.contributor.author Eivers, Bernard
dc.contributor.author O'Meara, Ciara M.
dc.contributor.author Lonergan, Patrick
dc.contributor.author Fair, Seán
dc.date.accessioned 2019-01-21T11:34:20Z
dc.date.available 2019-01-21T11:34:20Z
dc.date.issued 2018
dc.identifier.uri http://hdl.handle.net/10344/7502
dc.description peer-reviewed en_US
dc.description.abstract An equilibration period of approximately 3–4 h prior to semen cryopreservation is standard practice for maintaining membrane integrity and motility of bull sperm. However, a number of studies indicate that an overnight equilibration period prior to freezing results in improved post-thaw semen quality thus optimising pregnancy rates. The aim of this study was to assess the effect of increasing the equilibration time of bull semen up to 72 h before freezing on sperm quality parameters and calving rate (CR) following artificial insemination (AI) with frozen-thawed semen. The effect of holding semen at 4 °C for 6, 24, 48 or 72 h post dilution before freezing on subsequent post-thaw total and progressive motility (Experiment 1) and field fertility (n = 1640 inseminations, Experiment 2) of frozen-thawed semen was assessed. Equilibration time did not affect post-thaw total and progressive motility (P > 0.05). In addition, there was no effect (P > 0.05) of equilibration time on field fertility with a CR of 53.3, 50.5, 51.3 and 47.3 for the 6, 24, 48 and 72 h treatments, respectively. In conclusion, increasing the equilibration time of diluted bull semen from 6 to 72 h had no significant effect on CR, within the expected range of fertility outcomes, thus providing semen processing centres with flexibility in the time which semen can be held prior to freezing. en_US
dc.language.iso eng en_US
dc.publisher Elsevier en_US
dc.relation FECUND en_US
dc.relation.ispartofseries Theriogenology;108, pp. 217-222
dc.relation.uri https://doi.org/10.1016/j.theriogenology.2017.11.034
dc.rights This is the author’s version of a work that was accepted for publication in Theriogenology . Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Theriogenology, 2018, 108, pp. 217-222, en_US
dc.subject sperm en_US
dc.subject bovine en_US
dc.subject frozen-thawed semen en_US
dc.subject fertility en_US
dc.title Effect of increasing equilibration time of diluted bull semen up to 72 h prior to freezing on sperm quality parameters and calving rate following artificial insemination en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1016/j.theriogenology.2017.11.034
dc.contributor.sponsor IRC en_US
dc.contributor.sponsor Department of Agriculture, Food and the Marine en_US
dc.contributor.sponsor Teagasc en_US
dc.contributor.sponsor ERC en_US
dc.relation.projectid EBPPG/2014/60 en_US
dc.relation.projectid 312097 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2738527


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