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Recombinant β-defensin 126 promotes bull sperm binding to bovine oviductal epithelia

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dc.contributor.author Lyons, Alan
dc.contributor.author Narciandi, F.
dc.contributor.author Donnellan, E.
dc.contributor.author Romero-Aguirregomezcorta, Jon
dc.contributor.author O'Farrelly, Cliona
dc.contributor.author Lonergan, Patrick
dc.contributor.author Meade, Kieran G.
dc.contributor.author Fair, Seán
dc.date.accessioned 2019-01-21T09:23:52Z
dc.date.available 2019-01-21T09:23:52Z
dc.date.issued 2018
dc.identifier.uri http://hdl.handle.net/10344/7500
dc.description peer-reviewed en_US
dc.description.abstract Primate β-defensin 126 regulates the ability of spermatozoa to bind to oviductal epithelial cells in vitro. Bovine β-defensin 126 (BBD126) exhibits preferential expression in the cauda epididymis of the bull, but there have been few studies on its functional role in cattle. The aim of the present study was to examine the role of BBD126 in bull sperm binding to bovine oviductal epithelial cell (BOEC) explants. BBD126 has been shown to be highly resistant to the standard methods of dissociation used in other species and, as a result, corpus epididymal spermatozoa, which have not been exposed to the protein, were used to study the functional role of BBD126. Corpus epididymal spermatozoa were incubated with recombinant (r) BBD126 in the absence or presence of anti-BBD126 antibody. Addition of rBBD126 significantly enhanced the ability of epididymal spermatozoa to bind to BOEC explants (P < 0.05). Anti-BBD126 antibody blocked the BBD126-mediated increase in sperm binding capacity. Ejaculated spermatozoa, which are coated with native BBD126 protein but also a large number of seminal plasma proteins in vivo, were incubated with rBBD126 in the absence or presence of the anti-BBD126 antibody. Addition of rBBD126 significantly enhanced the ability of ejaculated spermatozoa to bind to BOEC explants (P < 0.05), whereas rBBD126 also reduced corpus sperm agglutination (P < 0.05). These results suggest that, similar to the role of its analogue in the macaque, spermatozoa with more BBD126 in their acrosome may represent spermatozoa with more oviduct binding capacity. en_US
dc.language.iso eng en_US
dc.publisher CSIRO Publishing en_US
dc.relation.ispartofseries Reproduction, Fertility and Development;30 (11), pp. 1472-1481
dc.relation.uri http://dx.doi.org/10.1071/RD17415
dc.subject sperm en_US
dc.subject oviduct en_US
dc.subject binding en_US
dc.subject β-defensins en_US
dc.title Recombinant β-defensin 126 promotes bull sperm binding to bovine oviductal epithelia en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1071/RD17415
dc.contributor.sponsor Irish Department of Agriculture, Food and the Marine en_US
dc.relation.projectid 11/S/104 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2868261


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