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Genomic identification, expression profiling, and functional characterization of CatSper channels in the bovine

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Show simple item record Johnson, Gillian P. English, Anne-Marie Cronin, Sinéad Hoey, David A. Meade, Kieran G. Fair, Seán 2019-01-18T11:20:22Z 2019-01-18T11:20:22Z 2017
dc.description peer-reviewed en_US
dc.description.abstract Cation channels of sperm (CatSper) are sperm-specific calcium channels with identified roles in the regulation of sperm function in humans, mice, and horses. We sought to employ a comparative genomics approach to identify conserved CATSPER genes in the bovine genome, and profile their expression in reproductive tissue. We hypothesized that CATSPER proteins expressed in bull testicular tissue mediates sperm hyperactivation and their rheotactic response in the reproductive tract of the cow. Bioinformatic analysis identified all four known CATSPER genes (CATSPER 1-4) in the bovine genome, and profiling by quantitative real-time polymerase chain reaction identified site-specific variation in messenger ribonucleic acid (mRNA) expression for all four genes along the reproductive tract of the bull. Using a novel antibody against CATSPER 1, protein expression was confirmed and localized to the principal piece of bull sperm, in agreement with what has been reported in other species. Subsequent treatment of bull sperm with either the calcium chelator ethylene glycol tetraacetic acid; mibefradil, a specific blocker of CatSper channels in human sperm; or CATSPER1 antibody all significantly inhibited caffeine-induced hyperactivation and the rheotactic response, supporting the concept that the calcium influx occurs via CatSper channels. Taken together, the work here provides novel insights into expression and function of CatSper channels in bull testicular tissue and in the function of ejaculated sperm. en_US
dc.language.iso eng en_US
dc.publisher Oxford University Press en_US
dc.relation 336882 en_US
dc.relation.ispartofseries Biology of Reproduction;97 (2), pp. 303-312
dc.rights This is a pre-copyedited, author-produced PDF of an article accepted for publication in Biology of Reproduction, 2018, 97 (2), pp. 303-312 following peer review. The version of record is available online at: en_US
dc.subject sperm en_US
dc.subject calcium en_US
dc.subject rheotaxis en_US
dc.subject hyperactivation en_US
dc.subject fertility en_US
dc.subject comparative reproduction en_US
dc.subject bull en_US
dc.title Genomic identification, expression profiling, and functional characterization of CatSper channels in the bovine en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US 2019-01-18T10:24:52Z
dc.identifier.doi 10.1093/biolre/iox082
dc.contributor.sponsor IRC en_US
dc.contributor.sponsor ERC en_US
dc.contributor.sponsor SFI en_US
dc.relation.projectid GOIPG/2014/1463 en_US
dc.relation.projectid 336882 en_US
dc.relation.projectid 13/ERC/L2864 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2727640
dc.internal.copyrightchecked Yes
dc.identifier.journaltitle Biology Of Reproduction
dc.description.status peer-reviewed

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