University of Limerick Institutional Repository

Platelet aggregometry assay for evaluating the effects of platelet agonists and antiplatelet compounds on platelet function in vitro

DSpace Repository

Show simple item record

dc.contributor.author Tsoupras, Alexandros
dc.contributor.author Zabetakis, Ioannis
dc.contributor.author Lordan, Ronan
dc.date.accessioned 2019-01-15T16:27:09Z
dc.date.available 2019-01-15T16:27:09Z
dc.date.issued 2019
dc.identifier.uri http://hdl.handle.net/10344/7490
dc.description peer-reviewed en_US
dc.description.abstract Platelet aggregometry assays are generally used for the analysis of platelet function but can also be adapted for further research and therapy focused applications. This method describes the procedures for the preparation of human platelet-rich plasma (PRP) and platelet-poor plasma (PPP) for the assessment of human platelet aggregation induced by agonists such as platelet-activating factor (PAF), thrombin, collagen, adenosine diphosphate (ADP), arachidonic acid, etc. This method can be applied in vitro to evaluate the aggregatory effects of these agonists and to assess the antiaggregatory effects of several bioactive antiplatelet agents (compounds of natural or pharmacological origin) in human PRP. This versatile method can be used in both basic and clinical research for the assessment of platelet aggregation (a major cardiovascular risk factor), platelet agonists, and inhibitors, in physiological or pathological conditions. This method can be adapted to assess platelet activity in postprandial and intervention studies ex vivo. en_US
dc.language.iso eng en_US
dc.publisher Elsevier en_US
dc.relation.ispartofseries MethodsX;6, pp. 63-70
dc.relation.uri https://doi.org/10.1016/j.mex.2018.12.012
dc.subject platelet aggregation en_US
dc.subject light transmission aggregometry en_US
dc.subject platelet-activating factor en_US
dc.subject thrombin en_US
dc.subject antiplatelet en_US
dc.subject agonist en_US
dc.subject in vitro en_US
dc.subject inhibitors en_US
dc.title Platelet aggregometry assay for evaluating the effects of platelet agonists and antiplatelet compounds on platelet function in vitro en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1016/j.mex.2018.12.012
dc.contributor.sponsor EI en_US
dc.relation.projectid IP/2017/0518; IP/2016/0488-Y en_US
dc.relation.projectid IP/2017/0596-Y; IP-2017-0635-Y en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search ULIR


Browse

My Account

Statistics