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Mesoporous matrices for the delivery of the broad spectrum bacteriocin, Nisin A

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dc.contributor.author Flynn, James
dc.contributor.author Mallen, Sarah
dc.contributor.author Durack, Edel
dc.contributor.author O'Connor, Paula M.
dc.contributor.author Hudson, Sarah P.
dc.date.accessioned 2018-12-05T09:55:55Z
dc.date.issued 2018
dc.identifier.uri http://hdl.handle.net/10344/7388
dc.description peer-reviewed en_US
dc.description.abstract Mesoporous matrices of different pore size and chemical composition were explored as potential delivery matrices for the broad spectrum bacteriocin, nisin A. The adsorption of nisin A onto two mesoporous silicates (MPS - SBA-15, MCM-41) and two periodic mesoporous organosilanes (PMO - MSE, PMO-PA) was examined. It was found that hydrophobic interactions dominated in the adsorption of this peptide to the matrices, lending the highest adsorption to MCM-41 with a small pore size of 2.8 nm. The hydrophobic ethylene-bridged MSE (6 nm pore) improved the loading and protection of nisin A from degradation by a non-specific protease pepsin, over un-functionalised SBA-15 which had a slightly larger pore size and less hydrophobic moieties. Nisin A did not adsorb onto an amine-functionalised PMO. Upon suspension in modified fasted state simulated gastric fluid (pH 1.6), the highest release of nisin A was observed from MCM-41, with a lower release from SBA-15 and MSE, with release following Higuchi release kinetics. No release was detected into modified fasted state simulated intestinal fluid (pH 6.5) but despite this, the suspended matrices loaded with nisin A remained active against Staphylococcus aureus. en_US
dc.language.iso eng en_US
dc.publisher Elsevier en_US
dc.relation 13CDA2122 en_US
dc.relation.ispartofseries Journal of Colloid and Interface Science;537, pp. 396-406
dc.relation.uri https://doi.org/10.1016/j.jcis.2018.11.037
dc.rights This is the author’s version of a work that was accepted for publication inJournal of Colloid and Interface Science . Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Colloid and Interface Science, 2018, 537, pp. 396-406, https://doi.org/10.1016/j.jcis.2018.11.037 en_US
dc.subject mesoporous matrices en_US
dc.subject Nisin A en_US
dc.subject controlled release en_US
dc.subject antimicrobial en_US
dc.subject periodic mesoporous organosilanes en_US
dc.subject simulated gastrointestinal fluid en_US
dc.subject bacteriocins en_US
dc.title Mesoporous matrices for the delivery of the broad spectrum bacteriocin, Nisin A en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1016/j.jcis.2018.11.037
dc.contributor.sponsor SFI en_US
dc.relation.projectid 13/CDA/2122 en_US
dc.date.embargoEndDate 2020-11-12
dc.embargo.terms 2020-11-12 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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