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Using real-time impedance-based assays to monitor the effects of fibroblast-derived media on the adhesion, proliferation, migration and invasion of colon cancer cells

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dc.contributor.author Dowling, Catríona M.
dc.contributor.author Herranz Ors, Carmen
dc.contributor.author Kiely, Patrick A.
dc.date.accessioned 2018-11-29T11:33:28Z
dc.date.available 2018-11-29T11:33:28Z
dc.date.issued 2014
dc.identifier.uri http://hdl.handle.net/10344/7366
dc.description peer-reviewed en_US
dc.description.abstract Increasing our knowledge of the mechanisms regulating cell proliferation, migration and invasion are central to understanding tumour progression and metastasis. The local tumour microenvironment contributes to the transformed phenotype in cancer by providing specific environmental cues that alter the cells behaviour and promotes metastasis. Fibroblasts have a strong association with cancer and in recent times there has been some emphasis in designing novel therapeutic strategies that alter fibroblast behaviour in the tumour microenvironment. Fibroblasts produce growth factors, chemokines and many of the proteins laid down in the ECM (extracellular matrix) that promote angiogenesis, inflammation and tumour progression. In this study, we use a label-free RTCA (real-time cell analysis) platform (xCELLigence) to investigate how media derived from human fibroblasts alters cancer cell behaviour. We used a series of complimentary and novel experimental approaches to show HCT116 cells adhere, proliferate and migrate significantly faster in the presence of media from human fibroblasts. As well as this, we used the xCELLigence CIM-plates system to show that HCT116 cells invade matrigel layers aggressively when migrating towards media derived from human fibroblasts. These data strongly suggest that fibroblasts have the ability to increase the migratory and invasive properties of HCT116 cells. This is the first study that provides real-time data on fibroblast-mediated migration and invasion kinetics of colon cancer cells. en_US
dc.language.iso eng en_US
dc.publisher Portland Press en_US
dc.relation.ispartofseries Bioscience Reports;34, art:e00126
dc.relation.uri http://www.bioscirep.org/
dc.subject cancer progression en_US
dc.subject colon cancer en_US
dc.subject real-time cell analysis en_US
dc.subject stromal environment en_US
dc.subject xCELLigence en_US
dc.title Using real-time impedance-based assays to monitor the effects of fibroblast-derived media on the adhesion, proliferation, migration and invasion of colon cancer cells en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.date.updated 2018-11-29T11:23:55Z
dc.description.version PUBLISHED
dc.identifier.doi 10.1042/BSR20140031
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 1573162
dc.internal.copyrightchecked Yes
dc.identifier.journaltitle Bioscience reports
dc.description.status peer-reviewed


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