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Hyperactivated stallion spermatozoa fail to exhibit a rheotaxis-like behaviour, unlike other species

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dc.contributor.author Romero-Aguirregomezcorta, Jon
dc.contributor.author Sugrue, Emer
dc.contributor.author Martínez-Fresneda, Lucía
dc.contributor.author Newport, David
dc.contributor.author Fair, Seán
dc.date.accessioned 2018-11-28T11:51:49Z
dc.date.available 2018-11-28T11:51:49Z
dc.date.issued 2018
dc.identifier.uri http://hdl.handle.net/10344/7359
dc.description peer-reviewed en_US
dc.description.abstract The journey of spermatozoa through the female genital tract is facilitated by rheotaxis, or the cell’s preference to swim against a flow, as well as thigmotaxis, the wall tracking behaviour, which guides them to the site of fertilisation. The aim of this study was to characterise the rheotactic and thigmotactic response of stallion sperm within a microfluidic channel. Stallion sperm rheotaxis was assessed within the microfluidic channel with regard to: (i) A range of flow velocities, (ii) Varying media viscosity and (iii) Sperm hyperactivation. Sperm distribution across the microfluidic channel was also studied and compared to human and ram sperm. Stallion sperm progressed furthest at a velocity range of 10–30 μm/s, with an optimum velocity of 20 μm/s. A flow viscosity of 2.5cP or greater reduced sperm rheotaxis (P < 0.05). Stallion sperm that were hyperactivated were unable to exhibit rheotaxis within the microfluidic channel, whereas, both hyperactivated human and ram sperm did exhibit positive rheotaxis under the same conditions. The number of sperm swimming near the microfluidic channel walls was higher than in the microfluidic channel centre (P < 0.05). This is the first study to illustrate that stallion sperm are rheotactically responsive and increasing viscosity reduces this response. We also demonstrated that sperm are predominantly inclined to swim along a surface and uniquely, hyperactivated stallion sperm are non-progressive and do not exhibit a rheotactic response unlike other species. en_US
dc.language.iso eng en_US
dc.publisher Nature Research en_US
dc.relation 16/IA/4474 en_US
dc.relation.ispartofseries Scientific Reports;8:16897
dc.relation.uri http://dx.doi.org/10.1038/s41598-018-34973-9
dc.subject hyperactivated en_US
dc.subject stallion en_US
dc.subject spermatozoa en_US
dc.subject rheotaxis-like behaviour en_US
dc.subject species en_US
dc.title Hyperactivated stallion spermatozoa fail to exhibit a rheotaxis-like behaviour, unlike other species en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1038/s41598-018-34973-9
dc.contributor.sponsor SFI en_US
dc.contributor.sponsor EI en_US
dc.relation.projectid SFI 16/IA/4474 en_US
dc.relation.projectid CF 2014 4364 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2865219


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