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The human mesenteric lymph node microbiome differentiates between Crohn’s disease and ulcerative colitis

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Show simple item record Kiernan, Miranda G. Coffey, Calvin J. McDermott, Kieran W. Cotter, Paul D. Cabrera-Rubio, Raul Kiely, Patrick A. Dunne, Colum P. 2018-11-08T15:26:55Z 2018-11-08T15:26:55Z 2019
dc.description peer-reviewed en_US
dc.description.abstract Background and Aims: Mesenteric lymph nodes are sites in which translocated bacteria incite and progress immunological responses. For this reason, understanding the microbiome of mesenteric lymph nodes in inflammatory bowel disease is important. The bacterial profile of Crohn’s disease mesenteric lymph nodes has been analysed using culture-independent methods in only one previous study. This study aimed to investigate the mesenteric lymph node microbiota from both Crohn’s disease and ulcerative colitis patients. Methods: Mesenteric lymph nodes were collected from Crohn’s disease and ulcerative colitis patients undergoing resection. Total DNA was extracted from mesenteric lymph nodes and assessed for the presence of bacterial DNA [16S]. All work was completed in a sterile environment using aseptic techniques. Samples positive for 16S DNA underwent next-generation sequencing, and the identity of bacterial phyla and species were determined. Results: Crohn’s disease mesenteric lymph nodes had a distinctly different microbial profile to that observed in ulcerative colitis. The relative abundance of Firmicutes was greater in nodes from ulcerative colitis patients, whereas Proteobacteria were more abundant in Crohn’s disease. Although species diversity was reduced in the mesenteric lymph nodes of patients with Crohn’s disease, these lymph nodes contained greater numbers of less dominant phyla, mainly Fusobacteria. Conclusion: This study confirms that there are distinct differences between the Crohn’s disease and ulcerative colitis mesenteric lymph node microbiomes. Such microbial differences could aid in the diagnosis of Crohn’s disease or ulcerative colitis, particularly in cases of indeterminate colitis at time of resection, or help explain their mechanisms of development and progression. en_US
dc.language.iso eng en_US
dc.publisher Oxford University Press en_US
dc.relation.ispartofseries Journal of Crohn's and Colitis; 13 (1), pp. 58-66
dc.subject inflammatory bowel disease en_US
dc.subject microbiome en_US
dc.subject mesenteric lymph nodes en_US
dc.title The human mesenteric lymph node microbiome differentiates between Crohn’s disease and ulcerative colitis en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1093/ecco-jcc/jjy136
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2895403

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