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Raman spectroscopy predicts the link between claw keratin and bone collagen structure in a mouse model of oestrogen deficiency

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dc.contributor.author Caraher, Clare
dc.contributor.author Sophocleous, Antonia
dc.contributor.author Beattie, Renwick J.
dc.contributor.author O'Driscoll, Olive M.
dc.contributor.author Cummins, Niamh M.
dc.contributor.author Brennan, Orlaith
dc.contributor.author O'Brien, Fergal J.
dc.contributor.author Ralston, Stuart H.
dc.contributor.author Bell, Steven E.J.
dc.contributor.author Towler, M.R.
dc.contributor.author Idris, Aymen I.
dc.date.accessioned 2018-10-08T11:34:26Z
dc.date.available 2018-10-08T11:34:26Z
dc.date.issued 2018
dc.identifier.uri http://hdl.handle.net/10344/7201
dc.description peer-reviewed en_US
dc.description.abstract Osteoporosis is a common disease characterised by reduced bone mass and an increased risk of fragility fractures. Low bone mineral density is known to significantly increase the risk of osteoporotic fractures, however, the majority of non-traumatic fractures occur in individuals with a bone mineral density too high to be classified as osteoporotic. Therefore, there is an urgent need to investigate aspects of bone health, other than bone mass, that can predict the risk of fracture. Here, we successfully predicted association between bone collagen and nail keratin in relation to bone loss due to oestrogen deficiency using Raman spectroscopy. Raman signal signature successfully discriminated between ovariectomised rats and their sham controls with a high degree of accuracy for the bone (sensitivity 89%, specificity 91%) and claw tissue (sensitivity 89%, specificity 82%). When tested in an independent set of claw samples the classifier gave 92% sensitivity and 85% specificity. Comparison of the spectral changes occurring in the bone tissue with the changes occurring in the keratin showed a number of common features that could be attributed to common changes in the structure of bone collagen and claw keratin. This study established that systemic oestrogen deficiency mediates parallel structural changes in both the claw (primarily keratin) and bone proteins (primarily collagen). This strengthens the hypothesis that nail keratin can act as a surrogate marker of bone protein status where systemic processes induce changes. en_US
dc.language.iso eng en_US
dc.publisher Elsevier en_US
dc.relation.ispartofseries BBA - Molecular Basis of Disease;1864 (2), pp. 398-406
dc.relation.uri https://doi.org/10.1016/j.bbadis.2017.10.020
dc.rights This is the author’s version of a work that was accepted for publication in BBA - Molecular Basis of Disease . Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in BBA - Molecular Basis of Disease, 2018, 1864 (2), pp. 398-406, https://doi.org/10.1016/j.bbadis.2017.10.020 en_US
dc.subject Raman spectroscopy en_US
dc.subject bone en_US
dc.subject collagen en_US
dc.subject keratin en_US
dc.subject osteoporosis en_US
dc.subject microCT en_US
dc.title Raman spectroscopy predicts the link between claw keratin and bone collagen structure in a mouse model of oestrogen deficiency en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1016/j.bbadis.2017.10.020
dc.contributor.sponsor IRC en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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