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Microfluidic chambers using fluid walls for cell biology

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Show simple item record Soitu, Cristian Feuerborn, Alexander Tan, Ann Na Walker, Henry Walsh, Pat A. Castrejón-Pita, Alfonso A. Cook, Peter R. Walsh, Ed J. 2018-10-04T11:26:15Z 2018-10-04T11:26:15Z 2018
dc.description peer-reviewed en_US
dc.description.abstract Many proofs of concept have demonstrated the potential of microfluidics in cell biology. However, the technology remains inaccessible to many biologists, as it often requires complex manufacturing facilities (such as soft lithography) and uses materials foreign to cell biology (such as polydimethylsiloxane). Here, we present a method for creating microfluidic environments by simply reshaping fluids on a substrate. For applications in cell biology, we use cell media on a virgin Petri dish overlaid with an immiscible fluorocarbon. A hydrophobic/ fluorophilic stylus then reshapes the media into any pattern by creating liquid walls of fluorocarbon. Microfluidic arrangements suitable for cell culture are made in minutes using materials familiar to biologists. The versatility of the method is demonstrated by creating analogs of a common platform in cell biology, the microtiter plate. Using this vehicle, we demonstrate many manipulations required for cell culture and downstream analysis, including feeding, replating, cloning, cryopreservation, lysis plus RT-PCR, transfection plus genome editing, and fixation plus immunolabeling (when fluid walls are reconfigured during use). We also show that mammalian cells grow and respond to stimuli normally, and worm eggs develop into adults. This simple approach provides biologists with an entrée into microfluidics. en_US
dc.language.iso eng en_US
dc.publisher PNAS: en_US
dc.relation MRK0108671 en_US
dc.relation.ispartofseries Proceedings of the National Academy of Sciences of the United States of America;115 (26), E5926–E5933
dc.subject microfluidics en_US
dc.subject fluid walls en_US
dc.subject tissue culture en_US
dc.subject sessile drops en_US
dc.subject interfacial tension en_US
dc.title Microfluidic chambers using fluid walls for cell biology en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1073/pnas.1805449115
dc.contributor.sponsor Iota Sciences Ltd. en_US
dc.contributor.sponsor Medical Research Council en_US
dc.contributor.sponsor ERC en_US
dc.relation.projectid MR/K010867/1 en_US
dc.relation.projectid 333848 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2869089

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