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Image-based tracking of anticancer drug-loaded nanoengineered polyelectrolyte capsules in cellular environments using a fast Benchtop Mid-Infrared (MIR) microscope

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dc.contributor.author Mouras, Rabah
dc.contributor.author Noor, Mohamed R.
dc.contributor.author Pastorino, Laura
dc.contributor.author Bagnoli, Enrico
dc.contributor.author Mani, Aladin
dc.contributor.author Durack, Edel
dc.contributor.author Antipov, Alexei
dc.contributor.author D’Autilia, Francesca
dc.contributor.author Bianchini, Paolo
dc.contributor.author Diaspro, Alberto
dc.contributor.author Soulimane, Tewfik
dc.contributor.author Silien, Christophe
dc.contributor.author Ruggiero, Carmelina
dc.contributor.author Tofail, Syed A.M.
dc.date.accessioned 2018-08-10T07:54:21Z
dc.date.available 2018-08-10T07:54:21Z
dc.date.issued 2018
dc.identifier.uri http://hdl.handle.net/10344/7049
dc.description peer-reviewed en_US
dc.description.abstract Drug delivery monitoring and tracking in the human body are two of the biggest challenges in targeted therapy to be addressed by nanomedicine. The ability of imaging drugs and micro-/nanoengineered drug carriers and of visualizing their interactions at the cellular interface in a labelfree manner is crucial in providing the ability of tracking their cellular pathways and will help understand their biological impact, allowing thus to improve the therapeutic efficacy. We present a fast, label-free technique to achieve high-resolution imaging at the mid-infrared (MIR) spectrum that provides chemical information. Using our custom-made benchtop infrared microscope using a high-repetition-rate pulsed laser (80 MHz, 40 ps), we were able to acquire images with subwavelength resolution (0.8 × λ) at very high speeds. As a proof-of-concept, we embarked on the investigation of nanoengineered polyelectrolyte capsules (NPCs) containing the anticancer drug, docetaxel. These NPCs were synthesized using a layer-by-layer approach built upon a calcium carbonate (CaCO3) core, which was then removed away with ethylenediaminetetraacetic acid. The obtained MIR images show that NPCs are attached to the cell membrane, which is a good step toward an efficient drug delivery. This has been confirmed by both three-dimensional confocal fluorescence and stimulated emission depletion microscopy. Coupled with additional instrumentation and data processing advancements, this setup is capable of video-rate imaging speeds and will be significantly complementing current super-resolution microscopy techniques while providing an unperturbed view into living cells. en_US
dc.language.iso eng en_US
dc.publisher American Chemical Society en_US
dc.relation 280804 en_US
dc.relation.ispartofseries ACS Omega; 3, pp. 6143-6150
dc.relation.uri http://dx.doi.org/http://dx.doi.org/10.1021/acsomega.7b01859
dc.subject drug delivery monitoring en_US
dc.subject tracking en_US
dc.subject human body en_US
dc.title Image-based tracking of anticancer drug-loaded nanoengineered polyelectrolyte capsules in cellular environments using a fast Benchtop Mid-Infrared (MIR) microscope en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1021/acsomega.7b01859
dc.contributor.sponsor ERC en_US
dc.contributor.sponsor EI en_US
dc.relation.projectid 280804 en_US
dc.relation.projectid IP20150404 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2868924


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