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Regulation of muscle protein synthesis in an in vitro cell model using ex vivo human serum

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dc.contributor.author Carson, Brian P.
dc.contributor.author Patel, Bajel
dc.contributor.author Amigo‐Benavent, Miryam
dc.contributor.author Pauk, Martina
dc.contributor.author Gujulla, Sunil Kumar
dc.contributor.author Murphy, Sylvia M.
dc.contributor.author Kiely, Patrick A.
dc.contributor.author Jakeman, Philip M.
dc.contributor.other Murphy-Tighe, Sylvia
dc.date.accessioned 2018-06-15T07:19:55Z
dc.date.issued 2018
dc.identifier.uri http://hdl.handle.net/10344/6899
dc.description peer-reviewed en_US
dc.description The full text of this article will not be available in ULIR until the embargo expires on the 1/04/2019
dc.description.abstract Human serum embodies the integrated systemic response to any condition or perturbation, which may regulate muscle protein synthesis (MPS). Conditioning of medium with human serum represents a physiologically relevant method of regulating MPS in vitro. The primary purpose of the study was the development of a model using ex vivo human serum to condition medium and regulate MPS in in vitro skeletal muscle cells. Four young healthy men reported to the laboratory after an overnight fast and were fed with 0.33 g (kg body mass)−1 whey protein. Blood samples were taken before (Fasted) and 60 min postprandial (Fed). Fully differentiated C2C12 skeletal muscle cells were nutrient and serum deprived for 1 h and subsequently treated with medium conditioned with Fasted or Fed ex vivo human serum (20%) for 4 h. The MPS was measured using the surface sensing of translation technique and activation of mTOR, P70S6K and 4EBP1 by Western blot. Fasted and fed ex vivo human serum increased MPS (P < 0.05). Although a strong effect (ƞ2 = 0.36) for increased MPS in Fed relative to Fasted was observed, this was not statistically significant (P > 0.05). Activation of mTOR, P70S6K and 4EBP1 was significantly increased after treatment with Fed compared with Fasted ex vivo human serum (P < 0.05). Here, we developed and optimized the conditions for culture of C2C12 skeletal muscle cells, measurement ofMPS and signalling inmedium conditioned by ex vivo human serum. Furthermore, the functionality of the model was demonstrated by comparison of the response to medium conditioned by Fasted and Fed ex vivo human serum. en_US
dc.language.iso eng en_US
dc.publisher Wiley and Sons Ltd en_US
dc.relation.ispartofseries Experimental Physiology;103 (6), pp. 783-789
dc.relation.uri https://doi.org/10.1113/EP086860
dc.rights This is the accepted version of the following article: Regulation of muscle protein synthesis in an in vitro cell model using ex vivo human serum Brian P. Carson, Bijal Patel, Miryam Amigo-Benavent, Martina Pauk, Sunil Kumar Gujulla, Sylvia M. Murphy, Patrick A. Kiely,Philip M.Jakeman Experimental Physiology 2018, 103 (6), pp. 783-789 which has been published in final form at https://doi.org/10.1113/EP086860 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. http://olabout.wiley.com/WileyCDA/Section/id-828039.html#terms
dc.subject muscle protein synthesis en_US
dc.subject serum en_US
dc.subject skeletal muscle en_US
dc.title Regulation of muscle protein synthesis in an in vitro cell model using ex vivo human serum en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1113/EP086860
dc.contributor.sponsor EI en_US
dc.relation.projectid TC20130001 en_US
dc.date.embargoEndDate 2019-04-01
dc.embargo.terms 2019-04-01 en_US
dc.rights.accessrights info:eu-repo/semantics/embargoedAccess en_US


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