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A multi-scale analysis of bull sperm methylome revealed both species peculiarities and conserved tissue-specific

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Show simple item record Perrier, Jean-Philippe Sellem, Eli Prézelin, Audrey Gasselin, Maxime Jouneau, Luc Piumi, François Adhami, Hala Al Weber, Michaël Fritz, Sébastien Boichard, Didier Le Danvic, Chrystelle Schibler, Laurent Jammes, Hélène Kiefer, Hélène 2018-06-14T11:09:38Z 2018-06-14T11:09:38Z 2018
dc.description peer-reviewed en_US
dc.description.abstract Background: Spermatozoa have a remarkable epigenome in line with their degree of specialization, their unique nature and different requirements for successful fertilization. Accordingly, perturbations in the establishment of DNA methylation patterns during male germ cell differentiation have been associated with infertility in several species.Background: Spermatozoa have a remarkable epigenResults: The quantification of DNA methylation at CCGG sites using luminometric methylation assay (LUMA) highlighted the undermethylation of bull sperm compared to the sperm of rams, stallions, mice, goats and men. Total blood cells displayed a similarly high level of methylation in bulls and rams, suggesting that undermethylation of the bovine genome was specific to sperm. Annotation of CCGG sites in different species revealed no striking bias in the distribution of genome features targeted by LUMA that could explain undermethylation of bull sperm. To map DNA methylation at a genome-wide scale, bull sperm was compared with bovine liver, fibroblasts and monocytes using reduced representation bisulfite sequencing (RRBS) and immunoprecipitation of methylated DNA followed by microarray hybridization (MeDIP-chip). These two methods exhibited differences in terms of genome coverage, and consistently, two independent sets of sequences differentially methylated in sperm and somatic cells were identified for RRBS and MeDIP-chip. Remarkably, in the two sets most of the differentially methylated sequences were hypomethylated in sperm. In agreement with previous studies in other species, the sequences that were specifically hypomethylated in bull sperm targeted processes relevant to the germline differentiation program (piRNA metabolism, meiosis, spermatogenesis) and sperm functions (cell adhesion, fertilization), as well as satellites and rDNA repeats. Conclusions: These results highlight the undermethylation of bull spermatozoa when compared with both bovine somatic cells and the sperm of other mammals, and raise questions regarding the dynamics of DNA methylation in bovine male germline. Whether sperm undermethylation has potential interactions with structural variation in the cattle genome may deserve further attention. While bull semen is widely used in artificial insemination, the literature describing DNA methylation in bull spermatozoa is still scarce. The purpose of this study was therefore to characterize the bull sperm methylome relative to both bovine somatic cells and the sperm of other mammals through a multiscale analysis. en_US
dc.language.iso eng en_US
dc.publisher BioMed Central en_US
dc.relation.ispartofseries BMC Genomics;19:404
dc.subject DNA methylation en_US
dc.subject sperm en_US
dc.subject cattle en_US
dc.subject satellite repeats en_US
dc.title A multi-scale analysis of bull sperm methylome revealed both species peculiarities and conserved tissue-specific en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1186/s12864-018-4764-0
dc.contributor.sponsor French National Research Agency en_US
dc.contributor.sponsor French Ministry of Higher Education en_US
dc.relation.projectid ANR-13-LAB3-0008-01 en_US
dc.relation.projectid ANR-11-INBS -0003 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US

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