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Spatial-domain filter enhanced subtraction microscopy and application to mid-IR imaging

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Show simple item record Kumbham, Mahendar Mouras, Rabah Mani, Aladin Daly, Susan O'Dwyer, Kevin Toma, Andrea Bianchini, Paolo Diaspro, Alberto Liu, Ning Tofail, Syed A.M. Silien, Christophe 2018-03-06T15:27:20Z 2018-03-06T15:27:20Z 2017
dc.description peer-reviewed en_US
dc.description.abstract We have experimentally investigated the enhancement in spatial resolution by image subtraction in mid-infrared central solid-immersion lens (c-SIL) microscopy. The subtraction exploits a first image measured with the c-SIL point-spread function (PSF) realized with a Gaussian beam and a second image measured with the beam optically patterned by a silicon it-step phase plate, to realize a centrally hollow PSF. The intense sides lobes in both PSFs that are intrinsic to the SIL make the conventional weighted subtraction methods inadequate. A spatial-domain filter with a kernel optimized to match both experimental PSFs in their periphery was thus developed to modify the first image prior to subtraction, and this resulted in greatly improved performance, with polystyrene beads 1.4 0.1 mu m apart optically resolved with a mid-1R wavelength of 3.4 mu m in water. Spatial-domain filtering is applicable to other PSF pairs, and simulations show that it also outperforms conventional subtraction methods for the Gaussian and doughnut beams widely used in visible and near-1R microscopy. (C) 2017 Optical Society of America en_US
dc.language.iso eng en_US
dc.publisher Optical Society of America en_US
dc.relation 280804 en_US
dc.relation.ispartofseries Optics Express;25 (12), pp. 1345-1352
dc.rights This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law. en_US
dc.subject contrast enhancement en_US
dc.subject confocal microscopy en_US
dc.subject resolution enhancement en_US
dc.subject vector beams en_US
dc.subject limit en_US
dc.title Spatial-domain filter enhanced subtraction microscopy and application to mid-IR imaging en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection ul_published_reviewed en_US 2018-03-06T15:07:00Z
dc.description.version PUBLISHED
dc.identifier.doi 10.1364/OE.25.013145
dc.contributor.sponsor ERC en_US
dc.contributor.sponsor SFI en_US
dc.contributor.sponsor IRC en_US
dc.contributor.sponsor INSPIRE
dc.relation.projectid 280804 en_US
dc.relation.projectid 13/TIDA/I2613 en_US
dc.relation.projectid 13/CDA/2221 en_US
dc.relation.projectid GOIPG/58/2013 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2719934
dc.internal.copyrightchecked Yes
dc.identifier.journaltitle Optics Express
dc.description.status peer-reviewed

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