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Equid herpesvirus 8: complete genome sequence and association with abortion in mares

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dc.contributor.author Garvey, Marie
dc.contributor.author Suárez, Nicolás M.
dc.contributor.author Kerr, Karen
dc.contributor.author Hector, Ralph D.
dc.contributor.author Moloney-Quinn, Laura
dc.contributor.author Arkins, Sean
dc.contributor.author Davison, Andrew J.
dc.contributor.author Cullinane, Ann
dc.date.accessioned 2018-02-15T14:22:26Z
dc.date.available 2018-02-15T14:22:26Z
dc.date.issued 2018
dc.identifier.uri http://hdl.handle.net/10344/6562
dc.description peer-reviewed en_US
dc.description.abstract Equid herpesvirus 8 (EHV-8), formerly known as asinine herpesvirus 3, is an alpha herpesvirus that is closely related to equid herpesviruses 1 and 9 (EHV-1 and EHV-9). The pathogenesis of EHV-8 is relatively little studied and to date has only been associated with respiratory disease in donkeys in Australia and horses in China. A single EHV-8 genome sequence has been generated for strain Wh in China, but is apparently incomplete and contains frame shifts in two genes. In this study, the complete genome sequences of four EHV-8 strains isolated in Ireland between 2003 and 2015 were determined by Illumina sequencing. Two of these strains were isolated from cases of abortion in horses, and were misdiagnosed initially as EHV-1, and two were isolated from donkeys, one with neurological disease. The four genome sequences are very similar to each other, exhibiting greater than 98.4% nucleotide identity, and their phylogenetic clustering together demonstrated that genomic diversity is not dependent on the host. Comparative genomic analysis revealed 24 of the 76 predicted protein sequences are completely conserved among the Irish EHV-8 strains. Evolutionary comparisons indicate that EHV-8 is phylogenetically closer to EHV-9 than it is to EHV-1. In summary, the first complete genome sequences of EHV-8 isolates from two host species over a twelve year period are reported. The current study suggests that EHV-8 can cause abortion in horses. The potential threat of EHV-8 to the horse industry and the possibility that donkeys may act as reservoirs of infection warrant further investigation. en_US
dc.language.iso eng en_US
dc.publisher Public Library of Science en_US
dc.relation.ispartofseries PLoS ONE;13 (2): e0192301
dc.relation.uri https://doi.org/10.1371/journal.pone.0192301
dc.subject Equid herpesvirus 8 en_US
dc.subject donkeys en_US
dc.subject genome en_US
dc.subject horses en_US
dc.subject abortion en_US
dc.title Equid herpesvirus 8: complete genome sequence and association with abortion in mares en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1371/journal.pone.0192301
dc.contributor.sponsor IRC en_US
dc.contributor.sponsor Medical Research Council en_US
dc.relation.projectid EBPPG/2014/62 en_US
dc.relation.projectid MC_UU_12014/3 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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