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Development of a platform for studying 3D astrocyte mechanobiology: compression of astrocytes in collagen gels

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dc.contributor.author Mulvihill, John J.E.
dc.contributor.author Raykin, Julia
dc.contributor.author Snider, Eric J.
dc.contributor.author Schildmeyer, Lisa A.
dc.contributor.author Zaman, Irsham
dc.contributor.author Platt, Manu O.
dc.contributor.author Kelly, Daniel J.
dc.contributor.author Ethier, Ross C.
dc.date.accessioned 2018-01-08T16:09:25Z
dc.date.issued 2017
dc.identifier.uri http://hdl.handle.net/10344/6413
dc.description peer-reviewed en_US
dc.description.abstract Glaucoma is a common optic neuropathy characterized by retinal ganglion cell death. Elevated intraocular pressure (IOP), a key risk factor for glaucoma, leads to significant biomechanical deformation of optic nerve head (ONH) cells and tissues. ONH astrocytes respond to this deformation by transforming to a reactive, proliferative phenotype, which has been implicated in the progression of glaucomatous vision loss. However, little is known about the mechanisms of this transformation. In this study, we developed a 3D collagen gel culture system to mimic features of ONH deformation due to elevated IOP. Compressive loading of astrocyte-seeded collagen gels led to cell alignment perpendicular to the direction of strain, and increased astrocyte activation, as assayed by GFAP, vimentin, and s100β levels, as well as MMP activity. This proof-of-concept study shows that this system has potential for studying mechanisms of astrocyte mechanobiology as related to the pathogenesis of glaucoma. Further work is needed to establish the possible interplay of mechanical stimulation, matrix properties, and hypoxia on the observed response of astrocytes. en_US
dc.language.iso eng en_US
dc.publisher Springer en_US
dc.relation.ispartofseries Annals of Biomedical Engineering; 46 (2), pp. 365–374
dc.relation.uri https://doi.org/10.1007/s10439-017-1967-5
dc.rights The original publication is available at www.springerlink.com en_US
dc.subject 3D culture model en_US
dc.subject astrocyte mechanobiology en_US
dc.subject glaucoma en_US
dc.subject mechanical conditioning en_US
dc.subject optic nerve head en_US
dc.title Development of a platform for studying 3D astrocyte mechanobiology: compression of astrocytes in collagen gels en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1007/s10439-017-1967-5
dc.contributor.sponsor IRC en_US
dc.contributor.sponsor National Science Foundation Graduate Research Fellowship en_US
dc.relation.projectid DGE-1148903 en_US
dc.date.embargoEndDate 2018-11-27
dc.embargo.terms 2018-11-27 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2737891


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