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Pico-litre sample introduction and acoustic levitation systems for time resolved protein crystallography experiments at XFELS

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Show simple item record Docker, Peter Morris, Robert Newton, Michael Dye, Elizabeth Kay, James Beale, John H. Axford, Danny Orville, Allen M. Stuart, Dave Leen, Gabriel 2017-12-21T10:20:19Z 2017-12-21T10:20:19Z 2017
dc.description peer-reviewed en_US
dc.description.abstract The system described in this work is a variant from traditional acoustic levitation first described by, Marzo et al [1]. It uses multiple transducers eliminating the requirement for a mirror surface, allowing for an open geometry as the sound from multiple transducers combines to generate the acoustic trap which is configured to catch pico litres of crystal slurries. These acoustic traps also have the significant benefit of eliminating potential beam attenuation due to support structures or microfluidic devices. Additionally they meet the need to eliminate sample environments when experiments are carried out using an X-ray Free Electron Lasers (XFEL) such as the Linac Coherent Light Source (LCLS) as any sample environment would not survive the exposure to the X-Ray beam. XFELs generate Light a billion times brighter than the sun. The application for this system will be to examine turn over in Beta lactamase proteins which is responsible for bacteria developing antibiotic resistance and therefore of significant importance to future world health. The system will allow for diffraction data to be collected before and after turnover allowing for a better understanding of the underling processes. The authors first described this work at Nanotech 2017 [2]. en_US
dc.language.iso eng en_US
dc.publisher IFSA Publishing, S.L. en_US
dc.relation.ispartofseries Sensors and Transducers;214 (7), pp. 39-45
dc.subject XFEL en_US
dc.subject pico-litre sample delivery en_US
dc.subject time resolved en_US
dc.subject protein crystallography en_US
dc.subject beta lactamase en_US
dc.subject acoustic levitation en_US
dc.title Pico-litre sample introduction and acoustic levitation systems for time resolved protein crystallography experiments at XFELS en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US

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