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Characterization of zinc amino acid complexes for zinc delivery in vitro using Caco-2 cells and enterocytes from hiPSC

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dc.contributor.author Sauer, Ann Katrin
dc.contributor.author Pfaender, Stefanie
dc.contributor.author Hagmeyer, Simone
dc.contributor.author Tarana, Laura
dc.contributor.author Mattes, Ann-Kathrin
dc.contributor.author Briel, Franziska
dc.contributor.author Küry, Sébastien
dc.contributor.author Boeckers, Tobias M.
dc.contributor.author Grabrucker, Andreas M.
dc.date.accessioned 2017-10-31T12:20:32Z
dc.date.available 2017-10-31T12:20:32Z
dc.date.issued 2017
dc.identifier.uri http://hdl.handle.net/10344/6209
dc.description peer-reviewed en_US
dc.description.abstract Zn is essential for growth and development. The bioavailability of Zn is affected by several factors such as other food components. It is therefore of interest, to understand uptake mechanisms of Zn delivering compounds to identify ways to bypass the inhibitory effects of these factors. Here, we studied the effect of Zn amino acid conjugates (ZnAAs) on the bioavailabilty of Zn. We used Caco-2 cells and enterocytes differentiated from human induced pluripotent stem cells from a control and Acroder- matitis enteropathica (AE) patient, and performed fluorescence based assays, protein biochemistry and atomic absorption spectrometry to characterize cellular uptake and absorption of ZnAAs. The results show that ZnAAs are taken up by AA transporters, leading to an intracellular enrichment of Zn mostly uninhibited by Zn uptake antagonists. Enterocytes from AE patients were unable to gain significant Zn through exposure to ZnCl2 but did not show differences with respect to ZnAAs. We conclude that ZnAAs may possess an advantage over classical Zn supplements such as Zn salts, as they may be able to increase bioavailability of Zn, and may be more efficient in patients with AE.   en_US
dc.language.iso eng en_US
dc.publisher Springer en_US
dc.relation.ispartofseries Biometals;30, pp. 643-661
dc.relation.uri http://rdcu.be/ugbB
dc.relation.uri http://dx.doi.org/10.1007/s10534-017-0033-y
dc.subject Zip4 en_US
dc.subject acrodermatitis enteropathica en_US
dc.subject absorption en_US
dc.subject gastro-intestinal en_US
dc.subject enterocyte en_US
dc.subject hIPSC en_US
dc.title Characterization of zinc amino acid complexes for zinc delivery in vitro using Caco-2 cells and enterocytes from hiPSC en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.date.updated 2017-10-31T12:10:18Z
dc.description.version PUBLISHED
dc.identifier.doi 10.1007/s10534-017-0033-y
dc.contributor.sponsor COST
dc.relation.projectid TD1304
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2718104
dc.internal.copyrightchecked Yes
dc.identifier.journaltitle Biometals
dc.description.status peer-reviewed


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