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Analysis and comparative genomics of R997, the first SXT/R391 integrative and conjugative element (ICE) of the Indian Sub-Continent

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dc.contributor.author Ryan, Michael P.
dc.contributor.author Armshaw, Patricia
dc.contributor.author O'Halloran, John A
dc.contributor.author Pembroke, Tony J.
dc.date.accessioned 2017-08-18T07:35:41Z
dc.date.available 2017-08-18T07:35:41Z
dc.date.issued 2017
dc.identifier.uri http://hdl.handle.net/10344/5998
dc.description peer-reviewed en_US
dc.description.abstract The aim of this study was to analyse R997, the first integrative and conjugative element (ICE) isolated from the Indian Sub-Continent, and to determine its relationship to the SXT/R391 family of ICEs. WGS of Escherichia coli isolate AB1157 (which contains R997) was performed using Illumina sequencing technology. R997 context was assessed by de novo assembly, gene prediction and annotation tools, and compared to other SXT/R391 ICEs. R997 has a size of 85 Kb and harbours 85 ORFs. Within one of the variable regions a HMS-1 β-lactamase resistance gene is located. The Hotspot regions of the element contains restriction digestion systems and insertion sequences. R997 is very closely related to the SXT-like elements from widely dispersed geographic areas. The sequencing of R997 increases the knowledge of the earliest isolated SXT/R391 elements and may provide insight on the emergence of these elements on the Indian sub-continent. en_US
dc.language.iso eng en_US
dc.publisher Nature Publishing Group en_US
dc.relation.ispartofseries Scientific Reports;7, article 8562
dc.subject SXT/R391 en_US
dc.title Analysis and comparative genomics of R997, the first SXT/R391 integrative and conjugative element (ICE) of the Indian Sub-Continent en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.date.updated 2017-08-17T10:05:39Z
dc.description.version PUBLISHED
dc.identifier.doi 10.1038/s41598-017-08735-y
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2719475
dc.internal.rssid 2719477
dc.internal.copyrightchecked Yes
dc.identifier.journaltitle Scientific Reports
dc.description.status peer-reviewed


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