University of Limerick Institutional Repository

The identification of bat species and their prey using PCR

DSpace Repository

Show simple item record

dc.contributor.advisor Breen, John
dc.contributor.advisor O'Grady, Audrey
dc.contributor.advisor Kiely, Patrick
dc.contributor.author Merrick, Heather
dc.date.accessioned 2017-08-17T15:10:23Z
dc.date.available 2017-08-17T15:10:23Z
dc.date.issued 2014
dc.identifier.uri http://hdl.handle.net/10344/5995
dc.description peer-reviewed en_US
dc.description.abstract This thesis describes two separate investigations: the first involves the use of polymerase chain reaction (PCR) to separate two cryptic species of bats; the second used PCR combined with cloning and sequencing to compare the diets of two bat species living in the same attic. This is the first Irish study to use PCR to analyse the diets of bats and separate between two cryptic species of pipistrelle bats, Pipistrellus pipistrellus and P. pygmaeus. Bat droppings were collected from an attic roost site in Birr, County Offaly and had to be assigned to the correct pipistrelle species prior to dietary analyses. PCR was carried out using speciesspecific primers and PCR products were cloned and sequenced. Results from both gel electrophoresis of PCR products and DNA sequencing indicated the presence of P. pygmaeus only. Hence, the diets of Plecotus auritus and P. pygmaeus were then compared. Arthropod specific primers were used in PCR to amplify short fragments (157bp) of generic arthropod DNA . Successful PCR products were cloned and sequenced. Sequence results showed that the P. pygmaeus foraged on Diptera (59%), Lepidoptera (36%) and Plecoptera (5%). Plecotus auritus had a similar diet and foraged on Diptera (53%), Lepidoptera (34%) and Plecoptera (6%) but also consumed members from the order Coleoptera (6%), and Orthoptera (1%) which are non-flying orders and may be captured by gleaning. Here it is suggested that the overlap in dietary habits is due to the abundance of food resources in this area. en_US
dc.language.iso eng en_US
dc.publisher University of Limerick en_US
dc.subject PCR en_US
dc.subject bat species en_US
dc.subject prey en_US
dc.title The identification of bat species and their prey using PCR en_US
dc.type info:eu-repo/semantics/masterThesis en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.type.supercollection ul_theses_dissertations en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search ULIR


Browse

My Account

Statistics