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In vitro characterisation of fresh and frozen sex-sorted bull spermatozoa

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dc.contributor.author Holden, Shauna A.
dc.contributor.author Murphy, Craig
dc.contributor.author Moreno, Juan F
dc.contributor.author Butler, Stephen T
dc.contributor.author Cromie, Andrew R.
dc.contributor.author Lonergan, Patrick
dc.contributor.author Fair, Seán
dc.date.accessioned 2017-07-26T10:18:29Z
dc.date.available 2017-07-26T10:18:29Z
dc.date.issued 2017
dc.identifier.uri http://hdl.handle.net/10344/5915
dc.description peer-reviewed en_US
dc.description.abstract This study sought to compare the in vitro characteristics of fresh and frozen non-sorted (NS) and sex-sorted (SS) bull spermatozoa. Experiment 1: Holstein–Friesian ejaculates (n = 10 bulls) were split across four treatments and processed: (1) NS fresh at 3 × 106 spermatozoa, (2) X-SS frozen at 2 × 106 spermatozoa, (3) X-SS fresh at 2 × 106 spermatozoa and (4) X-SS fresh at 1 × 106 spermatozoa. NS frozen controls of 20 × 106 spermatozoa per straw were sourced from previously frozen ejaculates (n = 3 bulls). Experiment 2: Aberdeen Angus ejaculates (n = 4 bulls) were split across four treatments and processed as: (1) NS fresh 3 × 106 spermatozoa, (2) Y-SS fresh at 1 × 106 spermatozoa, (3) Y-SS fresh at 2 × 106 spermatozoa and (4) X-SS fresh at 2 × 106 spermatozoa. Controls were sourced as per Experiment 1. In vitro assessments for progressive linear motility, acrosomal status and oxidative stress were carried out on Days 1, 2 and 3 after sorting (Day 0 = day of sorting. In both experiments SS fresh treatments had higher levels of agglutination in comparison to the NS fresh (P < 0.001), NS frozen treatments had the greatest PLM (P < 0.05) and NS spermatozoa exhibited higher levels of superoxide anion production compared with SS spermatozoa (P < 0.05). Experiment 1 found both fresh and frozen SS treatments had higher levels of viable acrosome-intact spermatozoa compared with the NS frozen treatments (P < 0.01). en_US
dc.language.iso eng en_US
dc.publisher CSIRO Publishing en_US
dc.relation.ispartofseries Reproduction, Fertility and Development;29 (7), pp. 1415-1425
dc.relation.uri http://dx.doi.org/10.1071/RD16086
dc.subject agglutination en_US
dc.subject bovine en_US
dc.subject gender bias en_US
dc.subject oxidative stress en_US
dc.subject sexed semen en_US
dc.title In vitro characterisation of fresh and frozen sex-sorted bull spermatozoa en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.date.updated 2017-07-26T09:23:46Z
dc.description.version ACCEPTED
dc.identifier.doi 10.1071/RD16086
dc.contributor.sponsor Department of Agriculture, Food and the Marine en_US
dc.relation.projectid 11/S/116 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2717273
dc.internal.copyrightchecked Yes
dc.identifier.journaltitle Reproduction Fertility And Development
dc.description.status peer-reviewed


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