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A comparison of semen diluents on the in vitro and in vivo fertility of liquid bull semen

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Show simple item record Murphy, Edel M. Murphy, Craig O'Meara, Ciara M. Dunne, Gemma Eivers, Bernard Lonergan, Patrick Fair, Seán 2017-07-26T08:02:00Z 2017
dc.description peer-reviewed en_US
dc.description.abstract The aim of this study was to assess the effect of semen diluent on calving rate (CR) following artificial insemination with liquid bull semen stored for up to 3 d postcollection. In experiment 1, the effect of storing liquid semen maintained at a constant ambient temperature in 1 of 7 different diluents [Caprogen (homemade), OptiXcell, BioXcell, BullXcell, INRA96, NutriXcell, or AndroMed (all commercially available)] on total and progressive motility was assessed on d 0, 1, 2, and 3 postcollection. In experiment 2, the field fertility of liquid semen diluted in Caprogen, BioXcell, or INRA96 and inseminated on d 1, 2, or 3 postcollection was assessed in comparison to frozen-thawed semen (total of n = 19,126 inseminations). In experiment 3, the effect of storage temperature fluctuations (4 and 18 C) on total and progressive motility following dilution in Caprogen, BioXcell, and INRA96 was assessed on d 0, 1, 2, and 3 postcollection. In experiment 1, semen stored in Caprogen, BioXcell, and INRA96 resulted in the highest total and progressive motility on d 1, 2, and 3 of storage compared with OptiXcell, BullXcell, NutriXcell, and AndroMed. In experiment 2, an effect of diluent on CR was found as semen diluted in BioXcell had a lower CR on d 1, 2, and 3 of storage (46.3, 35.4, and 34.0%, respectively) in comparison with Caprogen (55.8, 52.0, and 51.9%, respectively), INRA96 (55.0, 55.1, and 52.2%, respectively), and frozen-thawed semen (59.7%). Effects were found of parity, cow fertility sub-index, as well as the number of days in milk on CR. In experiment 3, when the storage temperature of diluted semen fluctuated between 4 and 18 C, to mimic what occurs in the field (nighttime vs. daytime), BioXcell had the lowest total and progressive motility in comparison to Caprogen and INRA96. In conclusion, diluent significantly affected sperm motility when stored for up to 3 d. Semen diluted in INRA96 resulted in a similar CR to semen diluted in Caprogen and to frozen-thawed semen, whereas that diluted in BioXcell resulted in a decreased CR. Consistent with this finding, semen diluted in BioXcell was less tolerant of temperature fluctuations than that stored in Caprogen or INRA96. Given that it can be used directly off the shelf, INRA96 may be a suitable alternative to Caprogen for the storage of liquid bull semen. en_US
dc.language.iso eng en_US
dc.publisher American Dairy Science Association en_US
dc.relation.ispartofseries Journal of Dairy Science;100 (2), pp. 1541-1554
dc.subject sperm en_US
dc.subject liquid semen en_US
dc.subject bovine en_US
dc.subject artificial insemination en_US
dc.subject calving rate en_US
dc.title A comparison of semen diluents on the in vitro and in vivo fertility of liquid bull semen en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US 2017-07-26T07:55:21Z
dc.description.version ACCEPTED
dc.identifier.doi 10.3168/jds.2016-11646
dc.contributor.sponsor IRC en_US
dc.contributor.sponsor Department of Agriculture, Food and the Marine en_US
dc.relation.projectid EBPPG/2014/60 en_US 2018-02-01
dc.embargo.terms 2018-02-01 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2699425
dc.internal.copyrightchecked Yes
dc.identifier.journaltitle Journal Of Dairy Science
dc.description.status peer-reviewed

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