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Comparative genomic identification and expression profiling of a novel β-defensin gene cluster in the equine reproductive tract

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Show simple item record Johnson, Gillian P. Lloyd, Andrew T O'Farrelly, Cliona Meade, Kieran G. Fair, Seán 2017-06-30T11:49:57Z 2017-06-30T11:49:57Z 2016
dc.description peer-reviewed en_US
dc.description.abstract β-defensins are small cationic proteins with potent immunoregulatory and antimicrobial activity. The number of genes encoding these peptides varies significantly between and within species but they have not been extensively characterised in the horse. Here, we describe a systematic search of the Equus caballus genome that identified a cluster of novel β-defensin genes on Chromosome 22, which is homologous to a cluster on bovine Chromosome 13. Close genomic matches were found for orthologs of 13 of the bovine genes, which were named equine β-defensins (eBD) 115, eBD116, eBD117, eBD119, eBD120, eBD122a, eBD123, eBD124, eBD125, eBD126, eBD127, eBD129 and eBD132. As expression of the homologous cluster in cattle was limited to the reproductive tract, tissue sections were obtained from the testis, caput, corpus and cauda epididymis and the vas deferens of three stallions and from the ovary, oviduct, uterine horn, uterus, cervix and vagina of three mares. Using a quantitative real-time polymerase chain reaction approach, each of the novel β-defensin genes showed distinct region-specific patterns of expression. Preferential expression in the caput epididymis of these novel defensins in the stallion and in the oviduct in the mare suggests a possible role in immunoprotection of the equine reproductive tract or in fertility. en_US
dc.language.iso eng en_US
dc.publisher CSIRO Publishing en_US
dc.relation.ispartofseries Reproduction Fertility and Development;28, (10), pp. 1499-1508
dc.subject antimicrobial peptide en_US
dc.subject equine genome en_US
dc.subject epididymis en_US
dc.subject fertility en_US
dc.subject mare en_US
dc.subject stallion en_US
dc.subject oviduct en_US
dc.title Comparative genomic identification and expression profiling of a novel β-defensin gene cluster in the equine reproductive tract en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US 2017-06-30T10:18:22Z
dc.description.version ACCEPTED
dc.identifier.doi 10.1071/RD14345
dc.contributor.sponsor IRC en_US
dc.contributor.sponsor Wellcome Trust en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2691548
dc.internal.copyrightchecked Yes
dc.identifier.journaltitle Reproduction Fertility And Development
dc.description.status peer-reviewed

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