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Investigation of the potential of hemp, pea, rice and soy protein hydrolysates as a source of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides

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Show simple item record Nongonierma, Alice B. Fitzgerald, Richard J. 2017-04-18T13:43:57Z 2017-04-18T13:43:57Z 2015
dc.description peer-reviewed en_US
dc.description.abstract Food protein hydrolysates contain peptide sequences with dipeptidyl peptidase IV (DPP-IV) inhibitory properties which may find use to improve serum glucose regulation in type 2 diabetics. Four plant protein isolates from hemp (H), pea (P), rice (R) and soy (S) were hydrolysed with three enzyme preparations: Corolase L10 (Cor), Promod 144MG (Prom) and Protamex (Prot). From the 12 hydrolysates generated after 4-h incubation, 7 yielded DPP-IV inhibitory activity <2.0 mg protein equivalent mL−1. Their IC50 values ranged from 0.73 ± 0.11 to 3.54 ± 0.24 mg dry weight (dw) hydrolysate mL−1 for the 4 h P_Prot and 4 h H_Cor hydrolysates, respectively. Simulated gastrointestinal digestion (SGID) of the intact proteins yielded DPP-IV IC50 values between 1.85 ± 0.34 and 4.50 ± 0.55 mg dw hydrolysate mL−1 for the R and H, respectively. The DPP-IV inhibitory potency of the 4 h hydrolysates subjected to SGID (DPP-IV IC50 ranging from 1.00 ± 0.42 to 3.83 ± 0.36 mg dw hydrolysate mL−1 for the SGID of the 4 h S_Prom and 4 h H_Prot, respectively) was generally higher than that of the corresponding intact protein subjected to SGID, with the exception of the SGID of R and that of the 4 h R_Prot hydrolysate which had similar IC50 values (P > 0.05). To our knowledge, this is the first study reporting that H and P protein hydrolysates inhibit DPP-IV in vitro. This study also demonstrates the potential benefit, in some instances, of hydrolysing plant protein substrates prior to oral ingestion with the view of releasing DPP-IV inhibitory peptides. en_US
dc.language.iso eng en_US
dc.publisher Springer en_US
dc.relation.ispartofseries Food Digestion;6 (1), pp. 19-29
dc.rights The original publication is available at en_US
dc.subject dipeptidyl peptidase IV inhibition en_US
dc.subject bioactive peptides en_US
dc.subject hemp en_US
dc.subject pea en_US
dc.subject rice en_US
dc.subject soy en_US
dc.subject simulated gastrointestinal digestion en_US
dc.title Investigation of the potential of hemp, pea, rice and soy protein hydrolysates as a source of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US 2017-04-11T09:12:06Z
dc.description.version ACCEPTED
dc.identifier.doi 10.1007/s13228-015-0039-2
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2705297
dc.internal.copyrightchecked Yes
dc.identifier.journaltitle Food Digestion
dc.description.status peer-reviewed

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