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Strategies for the release of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides in an enzymatic hydrolyzate of alpha-lactalbumin

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dc.contributor.author Nongonierma, Alice B.
dc.contributor.author Le Maux, Solène
dc.contributor.author Hamayon, Joël
dc.contributor.author Fitzgerald, Richard J.
dc.date.accessioned 2017-04-13T14:45:58Z
dc.date.issued 2016
dc.identifier.uri http://hdl.handle.net/10344/5716
dc.description peer-reviewed en_US
dc.description.abstract Bovine α-lactalbumin (α-La) contains numerous dipeptidyl peptidase IV (DPP-IV) inhibitory peptides sequences within its primary structure. In silico analysis indicated that the targeted hydrolysis of α-La with elastase should release DPP-IV inhibitory peptide sequences. An α-La isolate was hydrolysed with elastase under different conditions using an experimental design approach incorporating 3 factors (temperature, pH and enzyme to substrate ratio (E:S) ratio) at 2 levels. The hydrolyzate generated at pH 8.5, 50C, E:S 2.0% (w/w) (H9) displayed the lowest half maximal DPP-IV inhibitory concentration (IC50 = 1.20 ± 0.12 mg mL-1). Five α-La-derived DPP-IV inhibitory peptides (GY, GL, GI, NY and WL) predicted to be released in silico were identified by liquid-chromatography tandem mass spectrometry (LC-MS/MS) within H9 and its simulated gastrointestinal digestion (SGID) sample. This proof of concept study demonstrated the benefit of using a targeted approach combined with an experimental design in the generation of dietary protein hydrolyzates with DPP-IV inhibitory properties. en_US
dc.language.iso eng en_US
dc.publisher Royal Society of Chemistry en_US
dc.relation.ispartofseries Food and Function;7, pp. 3437-3443
dc.relation.uri http://dx.doi.org/10.1039/c6fo00239k
dc.rights Personal use of this material is permitted. Permission from Royal Society of Chemistry must be obtained for all other uses en_US
dc.subject response-surface methodology en_US
dc.subject protein-derived dipeptides en_US
dc.subject skin gelatin hydrolysate en_US
dc.subject induced diabetic-rats en_US
dc.title Strategies for the release of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides in an enzymatic hydrolyzate of alpha-lactalbumin en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.date.updated 2017-04-11T08:26:59Z
dc.description.version ACCEPTED
dc.identifier.doi 10.1039/c6fo00239k
dc.contributor.sponsor EI en_US
dc.relation.projectid TC2013-0001 en_US
dc.date.embargoEndDate 2017-07-04
dc.embargo.terms 2017-07-04 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 2640734
dc.internal.copyrightchecked Yes
dc.identifier.journaltitle Food & Function
dc.description.status peer-reviewed


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