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Inhibition of dipeptidyl peptidase IV (DPP-IV) by proline containing casein-derived peptides

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dc.contributor.author Nongonierma, Alice B.
dc.contributor.author Fitzgerald, Richard J.
dc.date.accessioned 2017-04-13T10:16:20Z
dc.date.available 2017-04-13T10:16:20Z
dc.date.issued 2013
dc.identifier.uri http://hdl.handle.net/10344/5708
dc.description peer-reviewed en_US
dc.description.abstract Dipeptides with a C terminal Pro inhibit dipeptidyl peptidase IV (DPP-IV), a key enzyme in incretin hormone processing. It was hypothesised that tri- and tetrapeptides with a proline at the C-terminus may also be DPP-IV inhibitors. Therefore, an in silico hydrolysis approach was used to release short(4 ≤ amino acids) C terminal Pro peptides from the individual caseins which constitute Pro rich substrates. This was achieved using theoretical digestion of caseins with a prolyl oligopeptidase activity. Fifteen peptides were subsequently selected for in vitro DPP-IV inhibitory analysis. Stability of these peptides to gastrointestinal enzymes was also evaluated in silico and the predicted breakdown peptides were assessed for their DPP-IV inhibitory and antioxidant potential. New DPP-IV inhibitors were identified, the most potent being Phe-Leu-Gln-Pro (IC50 65.3 +/- 3.5 mu M). A low in vitro antioxidant (2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging) activity was also associated with the peptides studied. The strategy presented highlights the utility of employing an in silico approach for the prediction of food-derived peptides with a potential role in glycaemic management for subsequent development of functional foods. (C) 2013 Elsevier Ltd. All rights reserved. en_US
dc.language.iso eng en_US
dc.publisher Elsevier en_US
dc.relation.ispartofseries Journal of Functional Foods;5 (4), pp. 1909-1917
dc.relation.uri http://doi.org/10.1016/j.jff.2013.09.012
dc.rights This is the author’s version of a work that was accepted for publication in Journal of Functional Foods. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Functional Foods, 5 (4), pp. 1909-1917, http://doi.org/10.1016/j.jff.2013.09.012 en_US
dc.subject dipeptidyl peptidase IV inhibitors en_US
dc.subject antioxidant en_US
dc.subject bioactive peptides en_US
dc.subject milk en_US
dc.subject proline en_US
dc.title Inhibition of dipeptidyl peptidase IV (DPP-IV) by proline containing casein-derived peptides en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.date.updated 2017-04-10T18:20:56Z
dc.description.version ACCEPTED
dc.identifier.doi 10.1016/j.jff.2013.09.012
dc.contributor.sponsor EI en_US
dc.relation.projectid CC20080001 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 1573843
dc.internal.copyrightchecked Yes
dc.identifier.journaltitle Journal Of Functional Foods
dc.description.status peer-reviewed


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