University of Limerick Institutional Repository

An optimised work-flow to reduce time-to-detection of carbapenemase-producing Enterobacteriaceae (CPE) using direct testing from rectal swabs

DSpace Repository

Show simple item record O'Connor, Ciara Kiernan, Miranda G. Finnegan, Cathriona O'Hara, Maureen Power, Lorraine O'Connell, Nuala H. Dunne, Colum P. 2016-11-17T12:10:52Z 2016
dc.description peer-reviewed en_US
dc.description.abstract Rapid detection of patients with carbapenem-producing Enterobacteriaceae (CPE) is essential for the prevention of nosocomial cross-transmission, allocation of isolation facilities and to protect patient safety. Here, we aimed to design a new laboratory work-flow, utilising existing laboratory resources, in order to reduce time-to-diagnosis of CPE. A review of the current CPE testing processes and of the literature was performed to identify a real-time commercial polymerase chain reaction (PCR) assay that could facilitate batch testing of CPE clinical specimens, with adequate CPE gene coverage. Stool specimens (210) were collected; CPE-positive inpatients (n=10) and anonymised community stool specimens (n=200). Rectal swabs (eSwab™) were inoculated from collected stool specimens and a manual DNA extraction method (QIAamp® DNA Stool Mini Kit) was employed. Extracted DNA was then processed on the Check-Direct CPE® assay. The three step process of making the eSwab™, extracting DNA manually and running the Check-Direct CPE® assay, took <5 minutes, 1 hour 30 minutes and 1 hour 50 minutes, respectively. It was time efficient with a result available in under 4 hours, comparing favourably with the existing method of CPE screening; average time-to-diagnosis of 48/72 hours. Utilising this CPE work-flow would allow a ‘same-day’ result. Antimicrobial susceptibility testing results, as is current practice, would remain a ‘next-day’ result. In conclusion, the Check-Direct CPE® assay was easily integrated into a local laboratory work-flow and could facilitate a large volume of CPE screening specimens in a single batch, making it cost-effective and convenient for daily CPE testing. en_US
dc.language.iso eng en_US
dc.publisher Taylor and Francis en_US
dc.relation.ispartofseries Bioengineered; 8 (3), pp. 217-224
dc.rights This is an Author's Original Manuscript of an article whose final and definitive form, the Version of Record, has been published in Bioengineered, 2016 © copyright Taylor & Francis, available online at: en_US
dc.subject carbapenemase-producing en_US
dc.subject check-direct CPE® en_US
dc.subject eSwab™ en_US
dc.subject Enterobacteriaceae en_US
dc.subject polymerase chain reaction en_US
dc.title An optimised work-flow to reduce time-to-detection of carbapenemase-producing Enterobacteriaceae (CPE) using direct testing from rectal swabs en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US 2016-11-17T11:55:44Z
dc.description.version ACCEPTED
dc.identifier.doi 10.1080/21655979.2016.1222335. 2017-08-17
dc.embargo.terms 2017-08-17 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 1645768
dc.internal.copyrightchecked Yes
dc.identifier.journaltitle Bioengineered
dc.description.status peer-reviewed

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search ULIR


My Account