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An optimised work-flow to reduce time-to-detection of carbapenemase-producing Enterobacteriaceae (CPE) using direct testing from rectal swabs

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dc.contributor.author O'Connor, Ciara
dc.contributor.author Kiernan, Miranda G.
dc.contributor.author Finnegan, Cathriona
dc.contributor.author O'Hara, Maureen
dc.contributor.author Power, Lorraine
dc.contributor.author O'Connell, Nuala H.
dc.contributor.author Dunne, Colum P.
dc.date.accessioned 2016-11-17T12:10:52Z
dc.date.issued 2016
dc.identifier.uri http://hdl.handle.net/10344/5350
dc.description peer-reviewed en_US
dc.description.abstract Rapid detection of patients with carbapenem-producing Enterobacteriaceae (CPE) is essential for the prevention of nosocomial cross-transmission, allocation of isolation facilities and to protect patient safety. Here, we aimed to design a new laboratory work-flow, utilising existing laboratory resources, in order to reduce time-to-diagnosis of CPE. A review of the current CPE testing processes and of the literature was performed to identify a real-time commercial polymerase chain reaction (PCR) assay that could facilitate batch testing of CPE clinical specimens, with adequate CPE gene coverage. Stool specimens (210) were collected; CPE-positive inpatients (n=10) and anonymised community stool specimens (n=200). Rectal swabs (eSwab™) were inoculated from collected stool specimens and a manual DNA extraction method (QIAamp® DNA Stool Mini Kit) was employed. Extracted DNA was then processed on the Check-Direct CPE® assay. The three step process of making the eSwab™, extracting DNA manually and running the Check-Direct CPE® assay, took <5 minutes, 1 hour 30 minutes and 1 hour 50 minutes, respectively. It was time efficient with a result available in under 4 hours, comparing favourably with the existing method of CPE screening; average time-to-diagnosis of 48/72 hours. Utilising this CPE work-flow would allow a ‘same-day’ result. Antimicrobial susceptibility testing results, as is current practice, would remain a ‘next-day’ result. In conclusion, the Check-Direct CPE® assay was easily integrated into a local laboratory work-flow and could facilitate a large volume of CPE screening specimens in a single batch, making it cost-effective and convenient for daily CPE testing. en_US
dc.language.iso eng en_US
dc.publisher Taylor and Francis en_US
dc.relation.ispartofseries Bioengineered; 8 (3), pp. 217-224
dc.relation.uri http://dx.doi.org/10.1080/21655979.2016.1222335
dc.rights This is an Author's Original Manuscript of an article whose final and definitive form, the Version of Record, has been published in Bioengineered, 2016 © copyright Taylor & Francis, available online at: http://dx.doi.org/10.1080/21655979.2016.1222335 en_US
dc.subject carbapenemase-producing en_US
dc.subject check-direct CPE® en_US
dc.subject eSwab™ en_US
dc.subject Enterobacteriaceae en_US
dc.subject polymerase chain reaction en_US
dc.title An optimised work-flow to reduce time-to-detection of carbapenemase-producing Enterobacteriaceae (CPE) using direct testing from rectal swabs en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.date.updated 2016-11-17T11:55:44Z
dc.description.version ACCEPTED
dc.identifier.doi 10.1080/21655979.2016.1222335.
dc.date.embargoEndDate 2017-08-17
dc.embargo.terms 2017-08-17 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 1645768
dc.internal.copyrightchecked Yes
dc.identifier.journaltitle Bioengineered
dc.description.status peer-reviewed


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