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Activation of the cAMP pathway induces RACK1-Dependent binding of β-Actin to BDNF promoter

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Show simple item record Neasta, Jeremie Fiorenza, Anna He, Dao-Yao Phamluong, Khanhky Kiely, Patrick A. 2016-09-30T14:47:58Z 2016-09-30T14:47:58Z 2016
dc.description peer-reviewed en_US
dc.description.abstract RACK1 is a scaffolding protein that contributes to the specificity and propagation of several signaling cascades including the cAMP pathway. As such, RACK1 participates in numerous cellular functions ranging from cell migration and morphology to gene transcription. To obtain further insights on the mechanisms whereby RACK1 regulates cAMP-dependent processes, we set out to identify new binding partners of RACK1 during activation of the cAMP signaling using a proteomics strategy. We identified β-actin as a direct RACK1 binding partner and found that the association between β-actin and RACK1 is increased in response to the activation of the cAMP pathway. Furthermore, we show that cAMP-dependent increase in BDNF expression requires filamentous actin. We further report that β-actin associates with the BDNF promoter IV upon the activation of the cAMP pathway and present data to suggest that the association of β-actin with BDNF promoter IV is RACK1-dependent. Taken together, our data suggest that β-actin is a new RACK1 binding partner and that the RACK1 and β-actin association participate in the cAMP-dependent regulation of BDNF transcription. en_US
dc.language.iso eng en_US
dc.publisher Public Library of Science en_US
dc.relation.ispartofseries PloS One;
dc.subject RACK1 en_US
dc.subject scaffolding protein en_US
dc.title Activation of the cAMP pathway induces RACK1-Dependent binding of β-Actin to BDNF promoter en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1371/journal.pone.0160948
dc.contributor.sponsor National Institutes of Health en_US
dc.contributor.sponsor SFI en_US
dc.relation.projectid 13/CDA/2228 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US

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