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Effect of colon cancer and surgical resection on skeletal muscle mitochondrial enzyme activity in colon cancer patients: a pilot study.

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dc.contributor.author Phillips, Bethan E.
dc.contributor.author Smith, Kenneth
dc.contributor.author Liptrot, Sarah
dc.contributor.author Atherton, Philip J.
dc.contributor.author Varadhan, Krishna
dc.contributor.author Rennie, Michael J
dc.contributor.author Larvin, Michael
dc.contributor.author Lund, Jonathan N.
dc.contributor.author Williams, John P
dc.date.accessioned 2016-04-19T14:29:19Z
dc.date.available 2016-04-19T14:29:19Z
dc.date.issued 2013
dc.identifier.citation Phillips BE, Smith K, Liptrot S, Atherton PJ, Varadhan K, Rennie MJ, Larvin M, Lund JN, Williams JP (2013) 'Effect of colon cancer and surgical resection on skeletal muscle mitochondrial enzyme activity in colon cancer patients: a pilot study'. Journal of cachexia, sarcopenia and muscle, 4 (1):71-77. en_US
dc.identifier.uri http://hdl.handle.net/10344/5025
dc.description peer-reviewed en_US
dc.description.abstract Background Colon cancer (CC) patients commonly suffer declines in muscle mass and aerobic function. We hypothesised that CC would be associated with reduced muscle mass and mitochondrial enzyme activity and that curative resection would exacerbate these changes. Methods We followed age-matched healthy controls and CC patients without distant metastasis on radiological imaging before and 6 weeks after hemi-colectomy surgery. Body composition was analysed using dual energy X-ray absorptiometry. Mitochondrial enzyme activity and protein concentrations were analysed in vastus lateralis muscle biopsies. Results In pre-surgery, there were no differences in lean mass between CC patients and age-matched controls (46.1+32.5 vs. 46.1+37.3 kg). Post-resection lean mass was reduced in CC patients (43.8+30.3 kg, P<0.01). When comparing markers of mitochondrial function, the following were observed: pyruvate dehydrogenase (PDH) activity was lower in CC patients pre-surgery (P<0.001) but normalized postresection and cytochrome c oxidase and pyruvate dehydrogenase E2 subunit protein expression were lower in CC patients pre-surgery and not restored to control values post-resection (P<0.001). Nuclear factor kappa-B, an inflammatory marker, was higher in CC patients pre-surgery compared to controls (P<0.01), returning to control levels post-resection. Conclusion Muscle mass was affected by surgery rather than cancer per se. PDH activity was however lower in cancer patients, suggesting that muscle mass and mitochondrial enzyme activity are not inextricably linked. This reduction in mitochondrial enzyme activity may well contribute to the significant risks of major surgery to which CC patients are exposed.                            en_US
dc.language.iso eng en_US
dc.publisher Wiley Open Access/Springer en_US
dc.relation.ispartofseries Journal of cachexia, sarcopenia and muscle;4 (1), pp. 71-77
dc.relation.uri http://dx.doi.org/10.1007/s13539-012-0073-7
dc.subject cancer en_US
dc.subject muscle en_US
dc.subject mitochondria en_US
dc.subject pyruvate dehydrogenase en_US
dc.title Effect of colon cancer and surgical resection on skeletal muscle mitochondrial enzyme activity in colon cancer patients: a pilot study. en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.date.updated 2016-04-19T13:56:13Z
dc.description.version PUBLISHED
dc.identifier.doi 10.1007/s13539-012-0073-7
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 1434529
dc.internal.copyrightchecked Yes
dc.description.status peer-reviewed


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